Both NK Sensitive and Resistant Mycoplasma Free Cell Lines Stimulate Release of NKCF
Studies on the NK lytic mechanism in our laboratory have produced evidence that natural killer cytotoxic factors (NKCF) may be the lytic mediators. Our model for the NK lytic mechanism proposes that after binding of the NK cell to the target, the target stimulates the effector to release NKCF (1,2). In support of this, we have found that effector cell populations enriched for NK cells in either human (3) or mouse (4) produce NKCF during coculture with NK sensitive tumor stimulator cells. The objective of this investigation was to determine if there is any NK specificity at the level of induction of NKCF release. Experiments were also carried out to determine if mycoplasma infection of tumor stimulator cells had any effect on the production of cytotoxic supernatants by murine or rat spleen cells. It was found that both NK sensitive and resistant mycoplasma free cell lines could stimulate release of NKCF. Some mycoplasma infected cell lines could generate cytotoxic supernatants, however, it is not clear whether the lytic activity is due to NKCF or some other agent.
KeywordsNatural Killer Natural Killer Cell Spleen Cell Mycoplasma Infection Cell Mediate Lysis
Unable to display preview. Download preview PDF.
- 2.S. C. Wright and B. Bonavida, Studies on the mechanism of natural killer cell-mediated cytotoxicity. IV. Interferon- induced inhibition of NK target cell susceptibility to lysis is due to a defect in their ability to stimulate release of natural killer cytotoxic factors (NKCF). J. Immunol. 130: 2965 (1983).PubMedGoogle Scholar
- 3.S. C. Wright, M. L. Weitzen, R. Kahle, G. A. Granger, and B. Bonavida, Studies on the mechanism of natural killer cell mediated cytotoxicity. II. Coculture of human PBL with NK- sensitive or resistant cell lines stimulates release of natural killer cytotoxic factors (NKCF) selectively cytotoxic to NK-sensitive target cells. J. Immunol. 130:2479 (1983).PubMedGoogle Scholar
- 8.S. C. Wright and B. Bonavida, Studies on the mechanism of natural killer cell mediated cytotoxicity. V. Lack of NK specificity at the level of induction of natural killer cytotoxic factors in cultures of human, murine, or rat effector cells stimulated with mycoplasma free cell lines. J. Immunol., in press (1984).Google Scholar
- 9.Y. Nast, In vitro studies on the mitogenic activity of mycoplas mal species toward lymphocytes. Rev. Infec. Pis. 4:5205 (1982).Google Scholar
- 10.B. C. Cole, J. C. Overall Jr., P. S. Lombard, and L. A. Glasgow, Induction of interferon in bovine and human lymphocyte cultures by mycoplasmas. Inf. and Immun. 14:88 (1976).Google Scholar