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Human NK Resistant Tumor Cell Lysis is Effected by IL-2 Activated Killer Cells

  • Elizabeth A. Grimm
  • Anthony A. Rayner
  • Debra J. Wilson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 187)

Abstract

In experimental animal systems, trarlsfer of cell populations immune to solid tumors has been successful in mediating the regression of such tumors. In human systems, much conflicting and confusing data has been reported attempting to define the analogous effector cells. Our approach to define human anti-tumor effectors cells was to avoid as many in vitro induced artifacts as possible, and to employ fresh (or cyropreserved, uncultured) surgical biopsy specimens of tumors as target cells, and to generate effectors in human serum with naturally occurring immune stimulants. We have found that normal or cancer patients1 PBL incubated for 2–3 days in preparations of IL-2, including purified recombinant IL-2 (RIL-2) acquires the unique capacity to kill human tumors in 4 hour chromium release assays (1–6). These lymphokine-activated killer cells (LAK) kill NK resistant fresh tumors with an efficiency and polyspecificity that has not been found reproducible in any other anti-tumor lytic system, and therefore provides yet another interesting and potentially biologically relevant system in which to examine the mechanism of cell mediated cytotoxicity. This report addresses some of the basic aspects of LAK killing, such as the killing efficiency of whole populations versus clones, kinetics of lysis as measured by the chromium release assay, correlation of chromium release with the expression of LAK effector cell markers, and morphology and phenotype of LAK effector cells, thereby setting the groundwork for further detailed studies of the mechanism of tumor killing by LAK.

Keywords

Chromium Release Allogeneic Tumor Chromium Release Assay Surgical Biopsy Specimen Effector Target Ratio 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Elizabeth A. Grimm
    • 1
  • Anthony A. Rayner
    • 1
  • Debra J. Wilson
    • 1
  1. 1.Surgical Neurology Branch, Natl. Inst. of Neurological and Communicative Disorders and StrokeNational Institutes of HealthBethesdaUSA

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