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Optical Properties of Human Skin

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The Science of Photomedicine

Part of the book series: Photobiology ((PB))

Abstract

Despite the many years since optical spectra from human skin were first obtained, only recently have quantitative models of cutaneous optics been applied. This chapter aims to present the optics of human skin conceptually and quantitatively, to examine the structures and pigments that modify cutaneous optics, and to discuss current research in this area and its applications to photomedicine. Introductory sections on the structure of skin and on optical phenomena in turbid media are included in addition to the general introduction below. This chapter does not offer an exhaustive review of all studies related to the optics of human skin, but attempts to include those reliable studies pertinent to its goals. The interested reader can find thorough and more historical reviews in (1–3).

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References

  1. Parrish, J. A., Anderson, R. R., Urbach, F., et al. (1978): UV-A: Biologic Effects of Ultraviolet Radiation With Emphasis on Human Responses to Longwave Ultraviolet. Plenum Press, New York.

    Google Scholar 

  2. Magnus, I. A. (1976): Dermatological Photobiology, pp. 11–22. Blackwell Scientific, Oxford.

    Google Scholar 

  3. Treagar, R. T. (1966): Physical Functions of the Skin, pp. 96–107. Academic Press, New York.

    Google Scholar 

  4. Parrish, J. A. (1975): Dermatology and Skin Care. McGraw-Hill, New York.

    Google Scholar 

  5. Montagna, W., and Parakkal, P. F. (1974): The Structure and Function of Skin, 3rd ed. Academic Press, New York.

    Google Scholar 

  6. Montagna, W., and Lobitz, W. (1964): The Epidermis. Academic Press, New York.

    Google Scholar 

  7. Fitzpatrick, T. B., Arndt, K. A., Clark, W. H., Jr., et al. (Eds.) (1971): Dermatology in General Medicine. McGraw-Hill, New York.

    Google Scholar 

  8. Malpighi, M. (1665): De Externo Tactus Organo Anatomica Observario. Naples.

    Google Scholar 

  9. Pechlin, J. (1677): De Habitu et Colore Aethiopum. Köln.

    Google Scholar 

  10. Thomson, M. L. (1955): The relative efficiency of pigment and horny layer thickness in protecting the skin of Europeans and Africans against solar ultraviolet radiation. J. Physiol. (Lond.) 127:236–246.

    CAS  Google Scholar 

  11. Everett, M. A., Yeargers, E., Sayre, R. M., et al (1966): Penetration of epidermis by ultraviolet rays. Photochem. Photobiol. 5:533–542.

    Article  PubMed  CAS  Google Scholar 

  12. Hardy, J. D., Hammell, H. T., and Murgatroyd D. (1956): Spectral transmittance and reflectance of excised human skin. J. Appl. Physiol. 9:257–264.

    PubMed  CAS  Google Scholar 

  13. Edwards, E. A., and Duntley, S. Q. (1939): The pigments and color of human skin. Am. J. Anat. 65:1–33.

    Article  Google Scholar 

  14. Kligman, A. (1969): Comments on the stratum corneum. In: The Biologic Effects of Ultraviolet Radiation (with Emphasis on the Skin), edited by F. Urbach, pp. 165–167. Pergamon Press, Oxford.

    Google Scholar 

  15. Baden, H. P., and Pearlman, C. (1964): Theeffects of ultraviolet light on protein and nucleic acid synthesis in the epidermis. J. Invest. Dermatol. 48:71–75.

    Google Scholar 

  16. Epstein, J. H., Fukuyama, K., and Fye, K. (1970): Effects of ultraviolet radiation on the mitotic cycle and DNA, RNA and protein synthesis in mammalian epidermis in vivo. Photochem. Photobiol. 12:57–65.

    Article  PubMed  CAS  Google Scholar 

  17. Parrish, J. A., Zaynoun, S., and Anderson, R. R. (1981): Cumulative effects of repeated subthreshold doses of ultraviolet radiation. J. Invest. Dermatol. 76:352–355.

    Article  Google Scholar 

  18. Langner, A., and Kligman, A. (1972): Tanning without sunburn with aminobenzoic acid type sunscreen. Arch. Dermatol. 106:338–343.

    Article  PubMed  CAS  Google Scholar 

  19. Kaidbey, K. H., and Kligman, A. M. (1978): Sunburn protection by longwave ultraviolet radiation-induced pigmentation. Arch. Dermatol. 114:46–48.

    Article  PubMed  CAS  Google Scholar 

  20. Scheuplein, R. J. (1964): A survey of some fundamental aspects of the absorption and reflection of light by tissue. J. Soc. Cosmet. Chem. 15:111–122.

    Google Scholar 

  21. Anderson, R. R., Hu, J. H., and Parrish, J. A. (1980): Optical radiation transfer in the human skin and application in in vivo remittance spectroscopy. In: Proceedings of the Symposium on Bioengineering and the Skin, Cardiff, Wales, July 19–21, 1979. MTP Press, London.

    Google Scholar 

  22. Jagger, J. (1967): Introduction to Research in Ultraviolet Photobiology. Prentice-Hall, Englewood Cliffs, N.J.

    Google Scholar 

  23. Kortüm, G. (1969): Reflectance Spectroscopy. Springer-Verlag, New York.

    Book  Google Scholar 

  24. Kubelka, P., and Münk, F. (1931): Ein Beitrag zür Optik der Farbanstriche. Z. technichse Physik 12:593–601.

    Google Scholar 

  25. Kubelka, P. (1948): New contributions to the optics of intensely light-scattering materials. I. J. Opt. Soc. Am. 38:448–457.

    Article  PubMed  CAS  Google Scholar 

  26. Kubelka, P. (1954): New contributions to the optics of intensely light-scattering materials. II: Nonhomogeneous layers. J. Opt. Soc. Am. 44:330–335.

    Article  Google Scholar 

  27. Schuster, A. (1905): Radiation through a foggy atmosphere. Astrophys. J. 21:1.

    Article  Google Scholar 

  28. Wendlandt, W. W., and Hecht, H. G. (1966): Reflectance Spectroscopy. Interscience, New York.

    Google Scholar 

  29. Atkins, J. T. (1969): Optical properties of turbid materials. In: The Biologic Effects of Ultraviolet Radiation (with Emphasis on the Skin), edited by F. Urbach, pp. 141–150. Pergamon Press, Oxford.

    Google Scholar 

  30. Hasselbalch, K. A. (1911): Quantitative Untersuchungen über die Absorption der menschlichen Haut von ultravioletten Strahlen. Skand. Arch. Physiol. 25:5–68.

    Article  Google Scholar 

  31. Bachem, A. (1929): Theultraviolettransparency ofthe various layers of human skin. Am. J. Physiol. 91:58–64.

    CAS  Google Scholar 

  32. Macht, D. I., Anderson, W. T., and Bell, F. K. (1928): The penetration of ultraviolet rays into live animal tissues. JAMA 90:161–165.

    Article  Google Scholar 

  33. Bachem, A., and Reed, C. I. (1930): The penetration of ultraviolet light through the human skin. Arch. Phys. Ther. 11:49–56.

    Google Scholar 

  34. Kirby-Smith, J. S., Blum, H. F., and Grady, H. G. (1942): Penetration of ultraviolet radiation into skin as a factor in carcinogenesis. J. Natl. Cane. Inst. 2:403–412.

    Google Scholar 

  35. Bachem, A., and Reed, C. I. (1929): The transparency of live and dead animal tissue to ultraviolet light. Am. J. Physiol. 90:600–606.

    CAS  Google Scholar 

  36. Lucas, N. S. (1930): The permeability of human epidermis to ultraviolet radiation. Biochem. J. 25:57–70.

    Google Scholar 

  37. Pathak, M. A. (1967): Photobiology of melanogenesis: biophysical aspects. In: Advances in Biology of the Skin, Vol. VIII, The Pigmentary System, edited by W. Montagna and F. Hu, pp. 397–420. Pergamon Press, Oxford.

    Google Scholar 

  38. Konev, S. V. (1967): Fluorescence and Phosphorescence of Proteins and Nucleic Acids. Plenum Press, New York.

    Book  Google Scholar 

  39. Fellner, M. J. (1976): Green autofluorescence of human epidermal cells. Arch. Dermatol. 112:667–670.

    Article  PubMed  CAS  Google Scholar 

  40. van der Leun, J. C. (1966): Ultraviolet erythema: a study on diffusion processes in human skin. Thesis, Utrecht, The Netherlands.

    Google Scholar 

  41. Morison, W. L., Parrish, J. A., Bloch, K. J., et al. (1979): In vivo effect of UVB on lymphocyte function. Br. J. Dermatol. 101:513–519.

    Article  PubMed  CAS  Google Scholar 

  42. Ortonne, J.-P., Claudy, A., Alario, A., et al. (1978): Impairment of thymus derived rosette forming cells during photochemotherapy (psoralen-UVA). Arch. Dermatol. Res. 262: 143–151.

    Article  PubMed  CAS  Google Scholar 

  43. Morison, W. L., Parrish, J. A., Anderson, R. R., et al. (1979): Sensitivity of mononuclear cells to UV radiation. Photochem. Photobiol. 29:1045–1047.

    Article  PubMed  CAS  Google Scholar 

  44. Hais, I. M., and Strych, A. (1969): Increase in urocanic acid concentration in human epidermis following insolation. Coll. Czech. Chem. Comm. 34:649–655.

    CAS  Google Scholar 

  45. Baden, H. P., and Pathak, M. A. (1967): The metabolism and function of urocanic acid in skin. J. Invest. Dermatol. 48:11–17.

    PubMed  CAS  Google Scholar 

  46. Weigand, D. A., Haygood, C., and Gaylor, J. R. (1974): Cell layersand density of Negro and Caucasian stratum corneum. J. Invest. Dermatol. 62:563–568.

    Article  PubMed  CAS  Google Scholar 

  47. Jacquez, J. A., Huss, J., McKeehan, W., et al (1956): Spectral reflectance of human skin in the region 0.7–2.6 µ. J. Appl. Physiol. 8:297–299.

    Google Scholar 

  48. Kuppenheim, H., and Heer, R. R., Jr. (1952): Spectral reflectance of white and Negro skin between 400 and 1000 mµ. J. Appl. Physiol. 4:800–806.

    PubMed  CAS  Google Scholar 

  49. Gates, R. R., and Zimmerman, A. A. (1953): Comparison of skin color with melanin content. J. Invest. Dermatol. 21:339–348.

    PubMed  CAS  Google Scholar 

  50. Szabo, G., Gerald, A. B., Pathak, M. A., et al. (1972): The ultrastructure of racial color differences in man. In: Pigmentation: Its Genesis and Control, edited by V. Riley, p. 23. Appleton-Century-Crofts, New York.

    Google Scholar 

  51. Quevedo, W. C., Jr., Fitzpatrick, T. B., Pathak, M. A., et al. (1974): Light and skin color. In: Sunlight and Man: Normal and Abnormal Photobiologic Responses, edited by M. A. Pathak, L. C. Harber, M. Seiji, et al. (T. B. Fitzpatrick, consulting ed.), pp. 165–194. Univ. of Tokyo Press, Tokyo.

    Google Scholar 

  52. Toda, K., Pathak, M. A., Parrish, J. A., et al. (1972): Alteration of racial differences in melanosome distribution in human epidermis after exposure to ultraviolet light. Nature [New Biol.] 236:143–145.

    CAS  Google Scholar 

  53. Kaidbey, K. H., Poh-Agin, P., Sayre, R. R., et al. (1979): Photoprotection by melanin—a comparison of black and Caucasian skin. J. Am. Acad. Dermatol. 1:249–260.

    Article  PubMed  CAS  Google Scholar 

  54. Olson, R. L., Gaylor, J., and Everett, M. A. (1973): Skin color, melanin, and erythema. Arch. Dermatol. 108:541–544.

    Article  PubMed  CAS  Google Scholar 

  55. Hausser, K. W., and Vahle, W. (1969): Sunburn and suntanning. In: The Biologic Effects of Ultraviolet Radiation (with Emphasis on the Skin), edited by F. Urbach, pp. 3–21. Pergamon Press, Oxford.

    Google Scholar 

  56. Zenisek, A., and Krahl, J. A. (1953): The occurrence of urocanic acid in sweat. Biochim. Biophys. Acta 12:479–484.

    Article  PubMed  CAS  Google Scholar 

  57. Everett, M. A., Anglin, J. H., and Bever, A. T. (1961): Ultraviolet-induced biochemical alterations in skin. Arch. Dermatol. 84:717–724.

    Article  PubMed  CAS  Google Scholar 

  58. Anglin, J. H., Jones, D. H., Bever, A. T., et al. (1966): The effect of ultraviolet light and thiol compounds on guinea pig skin histidase. J. Invest. Dermatol. 46:34–39.

    PubMed  CAS  Google Scholar 

  59. Cox, A. J., and Reaven, E. P. (1967): Histidine and keratohyalin granules. J. Invest. Dermatol. 49:31–34.

    PubMed  CAS  Google Scholar 

  60. Davis, E., and Landau, J. (1966): Clinical Capillary Microscopy. C C Thomas, Springfield, 111.

    Google Scholar 

  61. Findlay, G. H. (1970): Blue skin. Br. J. Dermatol. 83:127–134.

    Article  CAS  Google Scholar 

  62. Hanley, E. J., and De Witt, D. P. (1978): A physical model for detection of neonatal jaundice by multispectral skin reflectance analysis. In: Proceedings of the 6th New England Bioengineering Conference, edited by D. Jaron, pp. 346–349. Pergamon Press, New York.

    Google Scholar 

  63. Odell, G., Schaffer, R., and Simopoulos, A. (Eds.) (1974): Phototherapy in the Newborn,an Overview, National Academy of Sciences, Washington, D.C.

    Google Scholar 

  64. Noble, R. W., Brunori, M., Wyman, J., et al. (1967): Studies on the quantum yields of the photodissociation of carbon monoxide from hemoglobin and myoglobin. Biochemistry 6:1216.

    Article  PubMed  CAS  Google Scholar 

  65. Spartalian, K., Lang, G., and Yonetani, T. (1976): Low temperature photodissociation studies of ferrous hemoglobin and myoglobin complexes by Mossbauer spectroscopy. Biochim. Biophys. Acta 428:281–285.

    Article  PubMed  CAS  Google Scholar 

  66. Yoshida, S., and Orii, Y. (1977): Can hemo-CO complexes always be photodissociated? FEBS Lett. 79:129–132.

    Article  PubMed  CAS  Google Scholar 

  67. Anderson, R. R., Flicker, W., Roberts, J., et al. (1980): In vitro and in vivo photodissociation of carboxyhemoglobin in whole blood (abstract). In: Program and Abstracts, 8th Annual Meeting of the American Society for Photobiology, Colorado Springs, February 1980, p. 138.

    Google Scholar 

  68. Macht, D. I., Blackmann, S. S., and Kelley, E. B. (1923): An experimental contribution to the treatment of carbon monoxide poisoning. Proc. Soc. Exp. Biol. Med. 21:289–290.

    Google Scholar 

  69. Linder, E., Sakai, Y., and Paton, B. C. (1963): Experimental treatment of carbon monoxide poisoning by extracorporeal irradiation. Surg. Forum 14:277–278.

    PubMed  CAS  Google Scholar 

  70. Jacquez, J. A., Kuppenheim, H. F., Dimitroff, J. M., et al. (1956): Spectral reflectance of human skin in the region 235–700 mµ. J. Appl. Physiol. 8:212–214.

    Google Scholar 

  71. Goldzieher, J. W., Roberts, I. S., Rawls, W. B., et al. (1951): “Chemical” analysis of the intact skin by reflectance spectrophotometry. Arch. Dermatol. Syphilol. 64:533–548.

    Article  CAS  Google Scholar 

  72. Edwards, E. A., Finkelstein, N. A., and Duntley, S. Q. (1951): Spectrophotometryofliving human skin in ultraviolet range. J. Invest. Dermatol. 16:311–321.

    PubMed  CAS  Google Scholar 

  73. Sheard, C., and Brown, E. (1926): The spectrophotometric analysis of the color of the skin. Arch. Intern. Med. 38:816–831.

    Article  CAS  Google Scholar 

  74. Daniels, F., and Imbrie, J. D. (1958): Comparison between visual grading and reflectance measurements of erythema produced by sunlight. J. Invest. Dermatol. 30:295–301.

    PubMed  Google Scholar 

  75. Breit, R., and Kligman, A. M. (1969): Measurement of erythemal and pigmentary responses to ultraviolet radiation of different spectral qualities. In: The Biologic Effects of Ultraviolet Radiation (with Emphasis on the Skin), edited by F. Urbach, pp. 267–275. Pergamon Press, Oxford.

    Google Scholar 

  76. Mustakallio, K. K., and Korhonen, P. (1966): Monochromatic ultraviolet-photography in dermatology. J. Invest. Dermatol. 47:351–356.

    PubMed  CAS  Google Scholar 

  77. Gilchrest, B. A., Fitzpatrick, T. B., Anderson, R. R., et al. (1977): Localization of melanin pigmentation with Wood’s lamp. Br. J. Dermatol. 96:245–248.

    Article  PubMed  CAS  Google Scholar 

  78. Frank, L., Rapp, Y., and Bergman, L. V. (1962): An instrument for the objective measurement of erythema. J. Invest. Dermatol. 38:21–24.

    PubMed  CAS  Google Scholar 

  79. Tronnier, H. (1969) Evaluation and measurement of ultraviolet erythema. In: The Biologic Effects of Ultraviolet Radiation (with Emphasis on the Skin), edited by F. Urbach, pp. 255–265. Pergamon Press, Oxford.

    Google Scholar 

  80. Feather, J. W., Dawson, J. B., Barker, D. J., et al. (1980): A theoretical and experimental study of the optical properties of skin in vivo. In: Proceedings of the Symposium on Bioengineering and the Skin. MTP Press, International Medical Publishers, Cardiff.

    Google Scholar 

  81. Ballowitz, L., and Avery, M. E. (1970): Spectral reflectance of the skin. Biol. Neonate 15:348–360.

    Article  PubMed  CAS  Google Scholar 

  82. Bruce, R. A. (1978): Noninvasive estimation of bilirubin and hemoglobin oxygen saturation in the skin by reflection spectrophotometry. Ph.D. Thesis, Duke University, Durham, North Carolina.

    Google Scholar 

  83. Hannemann, R. E., De Witt, D. P., and Weichel, J. F. (1978): Neonatalserum bilirubin from skin reflectance. Pediatr. Res. 12:207–210.

    Article  PubMed  CAS  Google Scholar 

  84. Salthouse, T. N. (1958): Photography of the Negro skin. Med. Biol. Illus. 8:150.

    PubMed  CAS  Google Scholar 

  85. Marshall, R. J. (1976): Infrared and ultraviolet photography in a study of the selective absorption of radiation by pigmented lesions of skin. Med. Biol. Illus. 26:71–84.

    PubMed  CAS  Google Scholar 

  86. Wood, R. W. (1919): Secret communications concerning light rays. J. Physiol (Paris) 5e Serie IX (March 1919).

    Google Scholar 

  87. Caplan, R. M. (1967): Medical uses of the Wood’s lamp. JAMA 202:1035–1038.

    Article  PubMed  CAS  Google Scholar 

  88. Mitchell, J. S. (1938): The origin of the erythema curve and the pharmacological action of ultraviolet radiation. Proc. R. Soc. Lond. [Biol.] 126:241–246.

    Article  CAS  Google Scholar 

  89. Parrish, J. A. (1980): Action spectrum of phototherapy of psoriasis (abstract). J. Invest. Dermatol. 74:251.

    Google Scholar 

  90. Groves, G. A., Poh-Agin, P., and Sayre, R. M. (1979): In vitro and in vivo methods to define sunscreen protection, Australas J. Dermatol. 20:112–119.

    Article  PubMed  CAS  Google Scholar 

  91. Sayre, R. M., Poh-Agin, P., LeVee, G. J., et al. (1979): Acomparison of in vivo and in vitro testing of sunscreen formulas. Photochem. Photobiol. 29:559–566.

    Article  PubMed  CAS  Google Scholar 

  92. Blank, I. H., Ornellas, L., Anderson, R. R., et al. (1979): Observations on the substantivity of sunscreens (abstract). In: Program and Abstracts, 7th Annual Meeting of the American Society for Photobiology, Pacific Grove, California, June 1979, p. 127.

    Google Scholar 

  93. White, H. A. D., Anderson, R. R., Blank, I. H., et al. (1978): Enhancementoftransmission of optical radiation through human epidermis by topical applications (abstract). In: Program and Abstracts, 6th Annual Meeting of the American Society for Photobiology, Burlington, Vermont, June 1978, p. 68.

    Google Scholar 

  94. Anderson, R. R., Blank, I. H., and Parrish, J. A. (1979): Mechanisms of increased ultraviolet transmittance through human skin after topical applications (abstract). In: Program and Abstracts, 7th Annual Meeting of the American Society for Photobiology, Pacific Grove, California, June 1979, p. 141.

    Google Scholar 

  95. Anderson, R. R., LeVine, M. J., and Parrish, J. A. (1980): Selective modification of the optical properties of psoriatic vs. normal skin. In: Book of Abstracts, 8th International Photobiology Congress, Strasbourg, France, July 1980, p. 152.

    Google Scholar 

  96. Le Vine, M. J., White, H. A. D., and Parrish, J. A. (1979): Components of the Goeckerman regimen. J. Invest. Dermatol. 73:170–173.

    Article  PubMed  Google Scholar 

  97. Berns, M. W. (1974): Biological Microirradiation—Classical and Laser Sources. Prentice-Hall, Englewood Cliffs, N.J.

    Google Scholar 

  98. Goldman, L. (1973): Effects of new laser systems on the skin. Arch. Dermatol. 108:385–390.

    Article  PubMed  CAS  Google Scholar 

  99. Rosen, S., Noe, J. M., Kamat, B., et al. (1980): Healing of portwine stain after argon laser therapy. Arch. Dermatol., in press.

    Google Scholar 

  100. Noe, J. M., Barsky, S. H., Geer, D. E., et al. (1980): Portwine stains and the response to argon laser therapy: Successful treatment and the predictive role of color, age, and biopsy. Plast. Reconstr. Surg. 65:130–136.

    Article  CAS  Google Scholar 

  101. Rohsenow, W. M., and Hartnett, J. P. (1973): Handbook of Heat Transfer, pp. 3–59. McGraw-Hill, New York.

    Google Scholar 

  102. Greenwald, J., Rosen, S., Anderson, R. R., et al. (1981): Comparative histological studies of the tunable dye (at 577 nm) laser and argon laser: the specific vascular effects of the dye laser. J. Invest. Dermatol., 77:305–310.

    Article  PubMed  CAS  Google Scholar 

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Anderson, R.R., Parrish, J.A. (1982). Optical Properties of Human Skin. In: Regan, J.D., Parrish, J.A. (eds) The Science of Photomedicine. Photobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8312-3_6

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