High-Performance Thin-Layer Chromatography: A Quantitative Technique for Enantiomer Separation
Chiral stationary phases for thin-layer chromatography (TLC)/high-performance thin-layer chromatography (HPTLC) are a recent development compared to high-performance liquid chromatography (HPLC). Here the advantages offered by enantiomer resolution in TLC are described and the progress made in quantitative separations reviewed. Using test compounds, sensitivity levels and the possibility of quantitative determinations are investigated for N-(3,5-dinitrobenzoyl)-R-(-)-α-phenylglycine derivatised propylamino silica gel layers.
Unable to display preview. Download preview PDF.
- 4.M. Mack, H. E. Hauck and H. Herbert, J. Planar Chromatogr., 1: 304–308 (1988).Google Scholar
- 8.P. E. Wall, J. Planar Chromatogr., 2: 228–232 (1989).Google Scholar
- 10.U. B. Hezel in “HPTLC High Performance Thin-layer Chromatography”, A. Zlatkis and R.E. Kaiser, eds, J. Chromatography Library Vol. 9, Elsevier, Amsterdam, Ch. 8, 147–199 (1977).Google Scholar
- 11.C. F. Poole and S. Katib in “Quantitative Analysis Using Chromatographic Techniques”, E. Katz, ed, John Wiley, Chichester, Ch. 6, 240–243 (1987).Google Scholar
- 12.J. M. Finn in “Chromatographic Chiral Separations”, M. Zief and L.J. Crane, eds, Chromatographic Science Series Vol. 40, Marcel Dekker, New York, Ch. 3, 63 (1988).Google Scholar