Optimisation of Chiral Separation of Doxazosin Enantiomers by High-Performance Liquid Chromatography on a Second Generation α1-Acid Glycoprotein Column
The separation of the enantiomers of doxazosin (1-(4-amino-6,7-dimethyoxy-2quinazolinyl)-4-(1,4-bendioxan-2-yl-carbonyl) piperazine on a second generation α1-acid glycoprotein column is described. Following optimisation of pH and organic modifier concentration using a modified simplex procedure, an optimum mobile phase composition of phosphate buffer (0.035 M) at pH 7.25 and acetonitrile (87:13 v/v) was established. The method was linear over the range 2.6 to 520 ng (on column) with relative standard deviations of 4.0 and 4.9% for the first and second eluting enantiomers, respectively. The limit of detection for the first and second eluting enantiomers, using UV detection at 254 nm, was 1.3 and 1.7 ng, respectively, on column.
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- 3.J. C. Berridge, “Techniques for the Automated Optimisation of HPLC Separations”, John Wiley, New York, Ch. 4. 3, pp 62–69 (1985).Google Scholar
- 4.J. C. Berridge, “Techniques for the Automated Optimisation of HPLC Separations”, John Wiley, New York, 4.3:62–69; 6.2: 125–151 (1985).Google Scholar