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Optimisation of Chiral Separation of Doxazosin Enantiomers by High-Performance Liquid Chromatography on a Second Generation α1-Acid Glycoprotein Column

  • G. W. Ley
  • A. F. Fell
  • B. Kaye
Part of the Chromatographic Society Symposium Series book series (CSSS)

Summary

The separation of the enantiomers of doxazosin (1-(4-amino-6,7-dimethyoxy-2quinazolinyl)-4-(1,4-bendioxan-2-yl-carbonyl) piperazine on a second generation α1-acid glycoprotein column is described. Following optimisation of pH and organic modifier concentration using a modified simplex procedure, an optimum mobile phase composition of phosphate buffer (0.035 M) at pH 7.25 and acetonitrile (87:13 v/v) was established. The method was linear over the range 2.6 to 520 ng (on column) with relative standard deviations of 4.0 and 4.9% for the first and second eluting enantiomers, respectively. The limit of detection for the first and second eluting enantiomers, using UV detection at 254 nm, was 1.3 and 1.7 ng, respectively, on column.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • G. W. Ley
    • 1
  • A. F. Fell
    • 1
  • B. Kaye
    • 2
  1. 1.Department of Pharmaceutical Chemistry School of PharmacyUniversity of BradfordBradfordUK
  2. 2.Pfizer Central ResearchSandwich KentUK

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