Biochemical Substrates for the Development of the Matured Evoked Potential

  • Williamina A. Himwich
  • Jimmie M. Davis
  • Harish C. Agrawal


In our laboratory, we have for a number of years searched for ways of relating function to the biochemical development of the central nervous system [1, 2]. This approach was utilized very well for function (EEG) and neuroanatomy by Schadé [3] and for neurochemistry and neuroanatomy by Baxter, Schadé, and Roberts [4]. Since we were dissatisfied with the results of an attempt to correlate maturation of cortical EEG and amino-acid levels in young animals, we decided that a study of a functional system which has a clear-cut role and whose parts are readily available for rapid removal for chemical assay and for electrophysiological studies during the period of rapid postnatal development might give a more meaningful picture. The visual system in the rabbit offers such a convenient study. Our work with this system has started on the superior colliculi with measurements of the visual evoked potential, the biogenic amines, and the free-amino-acid content and will continue into histochemical studies. This paper is a preliminary report on the visual evoked potential, as a functional point of reference, and the changes in dopamine, norepinephrine, serotonin, glutamic acid, glutamine, GABA, aspartic acid, threonine, and alanine in the superior colliculi during development.


Glutamic Acid Aspartic Acid Biogenic Amine Superior Colliculus Biochemical Development 
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  1. 1.
    Himwich, W.A., and Petersen, J.C.: Correlation of chemical maturation of the brain in various species with neurologic behavior, in Masserman, J. H. (editor): Biological Psychiatry, Vol. 1, Grune and Stratton, New York, 1959, pp. 2–16.Google Scholar
  2. 2.
    Himwich, W.A., Petersen, J.C., and Graves, J. P.: Correlation of neurologic development and chemical maturation, in Wortis, J. (editor): Recent Advances in Biological Psychiatry, Vol. 3, Grune and Stratton, New York, 1961, pp. 218–226.Google Scholar
  3. 3.
    Schadé, J.P.: Origin of the spontaneous electrical activity of the cerebral cortex, in Wortis, J. (editor): Recent Advances in Biological Psychiatry, Vol. 2, Grune and Stratton, New York, 1960, pp. 23–42.Google Scholar
  5. 4.
    Baxter, C. F., Schade, J. P., and Roberts, E.: Maturational changes in cerebral cortex. II. Levels of glutamic acid decarboxylase, gamma-aminobutyric acid and some related amino acids, in Roberts, E., Baxter, C.F., Van Harreveld, A., Wiersma, C.A.C., Adey, W.R., and Killam, K. F. (editors): Inhibitions of the Nervous System and gamma-Aminobutyric Acid, Pergamon Press, London, 1960, pp. 213–220.Google Scholar
  6. 5.
    Bertler, A., Carlsson, A., and Rosengren, E.: A method for the fluorimetric determination of adrenaline and noradrenaline in tissues, Acta Physiol. Scand. 44: 273–292, 1958.Google Scholar
  7. 6.
    Carlsson, A., and Waldeck, B.: A fluorimetric method for the determination of dopamine (3-hydroxytyramine), Acta Physiol. Scand. 44: 293–298, 1958.Google Scholar
  8. 7.
    Bertler, A.: Effect of resperine on the storage of catechol amines in brain and other tissues, Acta Physiol. Scand. 51: 75–83, 1961.Google Scholar
  9. 8.
    Carlsson, A., and Lindqvist, M.: In-vivo decarboxylation of a -methyl DOPA and a -methyl metatyrosine, Acta Physiol. Scand. 54: 87–94, 1962.Google Scholar
  10. 9.
    Levin, E., Lovell, R.A., and Elliott, K.A.C.: The relation of gamma-aminobutyric acid to factor I in brain extracts, J. Neurochem. 7: 147–154, 1961.CrossRefGoogle Scholar
  11. 9a.
    Altman, J., and Malis, L. I.: An electrophysiological study of the superior colliculus and visual cortex, Exptl. Neurol. 5: 233–249, 1962.Google Scholar
  12. 10.
    Ellingson, R. J., and Wilcott, R.C.: Development of evoked responses in visual and auditory cortices of kittens, J. Neurophysiol. 23: 363–375, 1960.PubMedGoogle Scholar
  13. 11.
    Himwich, H. E., and Aprison, M. H.: The effect of age on cholinesterase activity of rabbit brain, in Waelsch, H. (editor): Biochemistry of the Developing Nervous System, Academic Press, New York, 1955, pp. 301–307.Google Scholar
  14. 12.
    Siou, C.: L’activité cholinesterasique dans les tubercules quadrijumeaux antérieurs au cours du développement post-natal chez le lapin, Arch. Anat. Microscop. Morphol. Exptl. 51: 287–324, 1962.Google Scholar
  15. 13.
    Hampson, J.L., Essig, C. F., McCauley, A., and Himwich, H. E.: Effects of di-isopropyl fluorophosphate (DFP) on electroencephalogram and cholinesterase activity, Electroencephalog. Clin, Neurophysiol. 2: 41–48, 1950.CrossRefGoogle Scholar
  16. 14.
    Talwar, G. P., Chopra, S. P., Goel, B. K., and D’Monte, B.: Correlation of the functional activity of the brain with metabolic parameters. III. Protein metabolism of the occipital cortex in relation to light stimulus, J. Neurochem. 13: 109–116, 1966.PubMedCrossRefGoogle Scholar
  17. 15.
    Häggendal, J., and Malmfors, T.: Identification and cellular localization of the catecholamines in the retina and the choroid of the rabbit, Acta Physiol. Scand. 64: 58–66, 1965.Google Scholar
  18. 16.
    Drujan, B. D., Borges, J. M. D., and Alvarez, N.: Relationship between the contents of adrenaline, noradrenaline and dopamine in the retina and its adaptational state, Life Sci. 4: 473–477, 1965.PubMedCrossRefGoogle Scholar
  19. 17.
    Utley, J. D.: Acetylcholinesterase and pseudo-cholinesterase in neural and non-neural tissue in the medial geniculate body of the cat, Biochem. Pharmacol. 15: 1–6, 1966.CrossRefGoogle Scholar
  20. 18.
    Volokhov, A.A., and Shilyagina, N.N.: Ontogenic development of function in cortical and subcortical parts of visual system, Zh. Evolyutsionnoi Biokhim. Fiziol. 1:84, 1965; Fed. Proc. 25 (2): T221 - T226, 1966.Google Scholar
  21. 19.
    Garrigan, O.W., and Chargaff, E.: Studies on the mucolipids and the cerebrosides of chicken brain during embryonic development, Biochem. Biophys. Acta 70: 452–464, 1963.CrossRefGoogle Scholar

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© Plenum Press 1967

Authors and Affiliations

  • Williamina A. Himwich
  • Jimmie M. Davis
  • Harish C. Agrawal

There are no affiliations available

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