Creatine Kinase and Intermediate Filaments in Cultured Mammalian Cells

  • Barry S. Eckert
  • Stephen J. Koons
  • C. Richard Zobel


Creatine kinase catalyzes the Lohmann reaction—the conversion of ADP and phosphorylcreatine to ATP and creatine. This reaction, essential in vertebrate muscle, has not been studied extensively in tissue other than muscle and brain. In this chapter, we summarize our immunofluorescence localization studies (Eckert et al.,1980) that show the association of creatine kinase with intermediate filaments in cultured rat kangaroo kidney epithelial cells (PTK1) and in cultured mouse uterine fibroblast cells (BALB/3T3). We also discuss the association of creatine kinase with the mitotic spindle of dividing cells (Koons et al., 1980, 1981).


Creatine Kinase Intermediate Filament Mitotic Spindle Creatine Kinase Activity Ehrlich Ascites Tumor Cell 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Barry S. Eckert
    • 1
  • Stephen J. Koons
    • 2
  • C. Richard Zobel
    • 2
  1. 1.Department of Anatomical SciencesState University of New York at BuffaloBuffaloUSA
  2. 2.Department of Biophysical SciencesState University of New York at BuffaloBuffaloUSA

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