Severity of Oxygen Free Radical Effects after Ischemia and Reperfusion in Intestinal Tissue and the Influence of Different Drugs
The intestinal tissue reacts to a temporary total occlusion of the superior mesenteric artery with a marked increase of lipid peroxidation as shown in previous experiments (Augustin and Lutz, 1988, Lutz and Augustin, 1989). This increase, as measured by the content of thiobarbituric acid reactive substances (TBARS), exceeds the reaction of the kidney to similar manipulations (e.g. Bird et al., 1988, Palier, 1988) severalfold: Whereas the increase of TBARS in the kidney is described as about 2–3 fold, we observed a more than 10 fold increase in intestinal tissue. Thus, the ischemic intestine is well suited as a model to test the effect of various drugs. According to different types and sources of oxygen free radicals in ischemic tissue, several therapeutic substances were chosen. Superoxide dismutase and catalase are scavengers of O2 and H2O2- Allopurinol is believed to act chiefly as xanthine oxidase inhibitor. An antibiotic therapy was chosen to restrain the inflammatory response and production of radicals via myeloperoxidase by neutrophiles.
KeywordsLipid Peroxide Superior Mesenteric Artery Xanthine Oxidase Oxygen Free Radical Intestinal Tissue
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