Heterogeneous Nadh Fluorescence During Post-Anoxic Reactive Hyperemia in Saline Perfused Rat Heart
Measurement of the autofluorescence of reduced pyridine nucleotide (NADH; 360nm excitation, 465nm emission), pioneered by Chance et al. (1962), not only enables the evaluation of cellular metabolic activity but also of cellular oxygen requirement (Chance, 1976). Steenbergen et al. (1977), using epicardial NADH fluorescence photography (Barlow and Chance, 1976), found that under steady-state conditions of low-flow of oxygenated perfusate as well as high-flow of poorly oxygenated perfusate heterogeneous NADH fluorescence patterns could be observed. In this study we investigated whether such patterns are also present during step-wise transitions of tissue oxygen content in the myocard such as occurs during reactive hyperemia.
KeywordsGrey Level Reactive Hyperemia Pyridine Nucleotide 465nm Emission Cellular Metabolic Activity
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