Comparison of Tyrode and Blood Perfused Working Isolated Rat Hearts
The isolated heart, perfused with an electrolyte solution (usually a modified Krebs-Henseleit or Tyrode buffer), is a widely used experimental model for the study of cardiac performance. The hearts can be perfused in a retrograde (Langendorff heart) or antegrade way (working heart). However, the oxygen supply of these hearts is very unphysiological, with very high PO2 values but a limited oxygen concentration of the perfusion medium. Thire exists a great deal of disagreement about the adequacy of oxygenation of this preparation, and one may conclude that it is on the brink of hypoxia (see review by Kreuzer, in press; Figulla et al., 1983; Opie, 1984; Robiolio et al., 1989).
KeywordsCoronary Flow Aortic Pressure Cardiac Performance Aortic Flow Supply Level
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