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Perfluorochemicals for Gas Transport and Improvement of Cell Cultures

  • Alastair T. King
  • Bernard J. Mulligan
  • Kenneth C. Lowe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 277)

Abstract

Much attention has focussed on the use of perfluorochemicals (PFCs) as organic oxygen carriers in biological systems (Sharma et al., 1987; Lowe, 1988a), Previous work has investigated the use of PFC emulsions as so-called “blood substitutes” and vehicles for tissue oxygenation (Lowe, 1988a). However, it is clear that great potential for such compounds also exists in other areas, especially in biotechnologically-important cell cultures. An additional attraction of using PFCs in cell culture systems is their immiscibility with aqueous media; in principle, they are therefore re-cycleable and recoverable, an obvious economic advantage. This paper considers the effects of PFCs and their emulsion components on both prokaryotic and eukaryotic cells and assesses their use for improving gas supply and regulating cellular growth.

Keywords

Plant Cell Culture Clostridium Perfringens Animal Cell Culture Klebsiella Oxytoca Human Lymphoblastoid Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Alastair T. King
    • 1
  • Bernard J. Mulligan
    • 2
  • Kenneth C. Lowe
    • 1
  1. 1.Department of ZoologyUniversity of NottinghamNottinghamUK
  2. 2.Department of BotanyUniversity of NottinghamNottinghamUK

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