Oxygen Transport by Pyridoxylated Polyhemoglobin Solution

  • G. Lenz
  • U. Bissinger
  • H. Benzing
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 277)


The principal requirement of any oxygen carrier is its ability to load and unload oxygen effectively. Oxygen-carrying solutions, however, do not necessarily contribute to peripheral oxygen delivery under all conditions. The efficacy of the perfluorochemical emulsion Fluosol-DA as a red cell substitute in acute anemia has been questioned (Gould et al., 1986). Even though stroma-free hemoglobin solutions (SFH) with elevated oxygen affinity (P50 < 20 mm Hg) have been shown to maintain effective oxygen delivery and physiological PO2 after total blood exchange (DeVenuto et al., 1979; Moss et al., 1984), doubt remains about the effectiveness of oxygen unloading at the tissue level after partial exchange transfusion with SFH and polyhemo-globin (PolyHb) preparations with increased oxygen affinity (Rosen et al., 1983). The physiological oxygen affinity (P50 27 mm Hg) of a pyridoxylated PolyHb (PolyHb-PPa) solution (Kothe et al., 1985) therefore takes on added significance.


Hemorrhagic Shock Hydroxyethyl Starch Exchange Transfusion Oxygen Affinity Hemoglobin Solution 
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  1. Daniels, F. H., McCabe, R. E., Leonard, E. F., 1984, The use of hemoglobin solutions, CRC Crit. Rev. Biomed. Engin., 9:315.Google Scholar
  2. DeVenuto, F., Zegna, A. I., Busse, K. R., 1979, Lyophilisation of crystalline hemoglobin solution and exchange transfusions with lyophilized, reconstituted hemoglobin, Surg. Gynecol. Obstet., 148:69.Google Scholar
  3. DeVenuto, F., Zuck, T. F., Zegna, A. I., Moores, W. Y., 1977, Characteristics of stroma-free hemoglobin prepared by crystallisation, J. Lab. Clin. Med., 89:509.Google Scholar
  4. Gould, S. A., Rosen, A. L., Seghal, L. R., Seghal, H. L., Langdale, L. A., Krause, L. M., Rice, C. L., Chamberlin, W. H., Moss, G. S., 1986, Fluosol-DA as a red-cell substitute in acute anemia, N. Engl. J. Med., 314:1653.PubMedCrossRefGoogle Scholar
  5. Gould, S. A., Rosen, A. L., Seghal, L. R., Seghal, H. L., Moss, G. S., 1987, Efficacy of polymerized pyridoxylated hemoglobin solution as an O2 carrier, Biomat. Art. Cells Art. Org., 15:359.Google Scholar
  6. Hobbhahn, J., Vogel, H., Kothe, N., Brendel, W., Peter, K., Jesch, F., 1985, Hemodynamics and oxygen transport after partial and total blood exchange with pyridoxylated polyhemoglobin in dogs, Acta Anaesthesiol. Scand., 29:537.PubMedCrossRefGoogle Scholar
  7. Keipert, P. E., Chang, T. M. S., 1984, Preparation and in vitro characteristics of a blood substitue based on pyridoxylated polyhemoglobin, Appl. Biochem. Biothechnol. 10:133.CrossRefGoogle Scholar
  8. Keipert, P. E., Chang, T. M. S., 1987, Oxygen affinity, viscosity, oncotic pressure, and long-term storage feasibility of pyridoxylated hemoglobin, Biomat. Art. Cells Art. Org., 15:369.Google Scholar
  9. Kothe, N., Eichentopf, B., Bonhard, K., 1985, Characterization of a modified, stroma-free hemoglobin solution as an oxygen-carrying plasma substitute, Surg. Gynecol. Obstet., 161:563.PubMedGoogle Scholar
  10. Lenz, G., Benzing, H., Junger, H., Bissinger, U., Feulner, R., Posininsky, H., Schlerf, S., 1983, Sauerstofftransportcharakterisika polymerisier-ter pyridoxalierter Hämoglobinlösungen in vitro und in vivo, Anaesthe-sist (Suppl.), 32:298.Google Scholar
  11. Lenz, G., 1988, Pryidoxalierte Polyhämoglobinlösung als sauerstofftransportierendes Volumenersatzmittel, Enke, Stuttgart.Google Scholar
  12. Messmer, K., Jesch, F., Schaff, J., Schoenberg, M., Pielsticker, K., Bonhard, K., 1978, Oxygen supply by stroma-free hemoglobin, in: “Blood Substitutes and Plasma Expanders,” G. A. Jamieson, T. J. Greenwalt, eds., Alan R. Liss, New York.Google Scholar
  13. Moss, G. S., DeWoskin, R., Rosen, A. L., Levine, H., Palani, C. K. (1976). Transport of oxygen and carbon dioxide by hemoglobin-saline solution in the red cell-free primate, Surg. Gynecol. Obstet., 142:357.PubMedGoogle Scholar
  14. Moss, G. S., Gould, S. A., Seghal, L. R., Seghal, H. L., Rosen, A. L., 1984, Hemoglobin solution — from tetramer to polymer, Surgery, 95:249.PubMedGoogle Scholar
  15. Rosen, A. L., Gould, S. A., Seghal, L. R., Seghal, H. L., Moss, G. S., 1983, Evaluation of efficacy of stroma free hemoglobin solutions, Prog. Clin. Biol. Res., 122:79.PubMedGoogle Scholar
  16. Tam, S. C., Blumenstein, J., Wong, J. T. F., 1978, Blood replacement in dogs by dextran-hemoglobin, Can. Biochem. J., 56:981.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • G. Lenz
    • 1
  • U. Bissinger
    • 1
  • H. Benzing
    • 2
  1. 1.Dept. of AnesthesiologyUniversity of TübingenFederal Republic of German
  2. 2.Dept. of PhysiologyUniversity of TübingenFederal Republic of Germany

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