Cerebral Cytochrome-C-Oxidase Copper Band Quantification in Perfluorocarbon Exchange Transfused Cats
Near infrared (IR) spectroscopy (NIRS) offers physicians and associate health care persons a potential means of monitoring changes in brain hemoglobin content and cerebral oxygenation noninvasively (Jobsis, 1977; Ferrari et al., 1989). The principles of NIRS have been explained previously (Jobsis, 1977; 1985). Briefly, IR light in the 700–1000 nm region is transmitted through soft and hard tissues of the head to provide a signal for spectrophotometric purposes. The intensity of the transmitted reflected light depends primarily on the wavelength, scattering effects, and upon NIRS absorption by the most abundant chromophore, i.e. the heme of hemoglobin. It has been suggested that cytochrome-c-oxidase (Cyt a,a3) redox band, previously described “in vitro” (Wharton and Tzagoloff, 1964), contributes to the absorption band providing a means for “in vivo” quantification (Jobsis, 1977; Brazy and Lewis, 1986; Wyatt et al. 1986).
KeywordsCerebral Oxygenation Near Infrared Spectroscopy Exchange Transfusion Somatosensory Evoke Potential Diffuse Transmittance
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