Advertisement

Special Problems of NMR in H2O Solution

  • A. G. Redfield
Part of the NATO ASI Series book series (NSSA, volume 107)

Abstract

Most biochemical NMR is performed in aqueous solutions, most often in D2O containing typically 1% proton impurity as HDO, but also quite often in 80–100% H2O. The latter is necessary if you want to observe exchangeable protons in a macromolecule, or if for some reason replacement of H2O by D2O is impractical. The large narrow HDO or H2O signal, equivalent to up to 100 molar proton concentration, presents various problems which will be outlined here, together with partial solutions.

Keywords

Free Induction Decay Solvent Saturation Pulse Method Selective Pulse Water Magnetization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. G. Redfield and R. K. Gupta. Adv. Magn. Reson. 5, 81 (1971).Google Scholar
  2. 2.
    A. G. Redfield. NMR: Basic Principles and Progress 13, 152 (1976).Google Scholar
  3. 3.
    A. G. Redfield. Methods Enzymol. 49, 253 (1978).PubMedCrossRefGoogle Scholar
  4. 4.
    J. D. Stoesz, A. G. Redfield, and D. Malinowski. FEBS Letters 9, 320 (1978).CrossRefGoogle Scholar
  5. 5.
    D. I. Hoult and R. E. Richards. J. Magn. Reson. 22, 561 (1976).CrossRefGoogle Scholar
  6. 6.
    K. Roth, B. J. Kimber, and J. Feeney. J. Magn. Reson. 41, 302 (1980).CrossRefGoogle Scholar
  7. 7.
    P. Plateau, C. Dumas, and M. Gueron. J. Magn. Reson. 54, 46 (1983).CrossRefGoogle Scholar
  8. 8.
    P. J. Hore. J. Magn. Reson. 55, 285 (1983).Google Scholar
  9. 9.
    G. M. Clore, B. J. Kimber, and A. M. Gronnenborn. J. Magn. Reson. 54, 170 (1983).CrossRefGoogle Scholar
  10. 10.
    A. G. Redfield. Chem. Fhys. Lett. 96, 537 (1983).CrossRefGoogle Scholar
  11. 11.
    C. Arus, M. Barany, W. M. Westler, and J. L. Markley. J. Magn. Reson. 57, 519 (1984).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • A. G. Redfield
    • 1
  1. 1.Department of BiochemistryBrandeis UniversityWalthamUSA

Personalised recommendations