Special Problems of NMR in H2O Solution
Most biochemical NMR is performed in aqueous solutions, most often in D2O containing typically 1% proton impurity as HDO, but also quite often in 80–100% H2O. The latter is necessary if you want to observe exchangeable protons in a macromolecule, or if for some reason replacement of H2O by D2O is impractical. The large narrow HDO or H2O signal, equivalent to up to 100 molar proton concentration, presents various problems which will be outlined here, together with partial solutions.
KeywordsFree Induction Decay Solvent Saturation Pulse Method Selective Pulse Water Magnetization
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