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Facilitation of Ion Permeability of Bilayer Membranes and their Phase Transition

  • Israel R. Miller

Abstract

The rate of relase of Tl+ from phospholipid vesicles of different composition was measured by pulse polarography as a function of temperature or in the presence of valinomycin, tetraphenyl boron (TPB) or dipicylamine (DPA) as transport facilitators. The release from pure dipalmitoyl phosphatidylcholine (DPPC) vesicles increased abruptly around the pretransition temp-earture. The steepness of the increase decreased with the width of the transition peak. Valinomycin TPB (tetraphenyl boron) and DPA- (dipicryl amine) facilitate release of Tl+ from unilamellar vesicles above their phase transition temperature with a first order release rate constant. They do not facilitate release below the phase transition. Bursts of release were observed upon their addition to the vesicles but after annealing, which was completed within less than a minute, the vesicles were re-sealed. The rate of phase transitions, their reversibility or irreversibility and hysteresis with concomitant existence of metastable states, will be discussed.

Keywords

Phase Transition Temperature Liquid Crystalline State Cooperative Unit Tetraphenyl Boron Phenyl Boron 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Benz, J., Duzgunes, N. and Nir, S. (1983) Biochemistry 22:3320–3330CrossRefGoogle Scholar
  2. Blok, M.C., van Deenen, L.M., and de Gier, J. (1976) Biochim. Biophys. Acta 433:1–12Google Scholar
  3. Bunov, M.R. (1979) Biochim. Biophys. Acta 74:542–546Google Scholar
  4. Ciani, S., Laprade, R., Eisenman, G., and Szabo, G. (1973) J. Memb. Biol. 11:255–292Google Scholar
  5. Davis, P.J., Coolbear, K.P., and Keough, K.M.W. (1980) Can. J. Biochem. 54:851–859Google Scholar
  6. Deamer, D.D. and Bangham, A.D. (1976) Biochim. Biophys. Acta 443:629–634Google Scholar
  7. Eisenman, G., Kranse, S., and Ciani, S. (1975) Ann. NY Acad. Sci 264:34–60Google Scholar
  8. Elamrani, K., and Blume, A. (1984) Biochemistry 22:3305–3311CrossRefGoogle Scholar
  9. Estep, T.N., Calhoun, W.I., Barneholz, Y., Bilton, R., Shipley, G.G., and Thompson, T.E. (1980) Biochemistry 19:20–24PubMedCrossRefGoogle Scholar
  10. Everett, D.H. (1978) Some biological implications of hysteresis in ions on macromolecular and biological systems, Ed. D.H. Everett and B. Vincent, Colston Papers no. 29, pp. 129–200Google Scholar
  11. Freire, E., Bach, D., Correa-Freire, M., Miller, I.R., and Barenholz, Y., (1980) Biochemistry 19:3662–3665PubMedCrossRefGoogle Scholar
  12. Gutknecht, J. (1983) Biochim. Biophys. Acta 735:185–188Google Scholar
  13. Hinz, H.J., and Sturtevan, J.M. (1972) J. Biol. Chem. 247:6071–6075Google Scholar
  14. Hladky, S.B. (1979) The carrier mechanism in current topics. In: Membranes and Transport, 12:53–164. Eds: F. Bonner and A. Kleinzeller. Academic PressGoogle Scholar
  15. Klein, R.A. (1982) Quart. Rev. of Biophys. 15:667–757CrossRefGoogle Scholar
  16. Lauger, P. and Stark, G. (1970) Biochim. Biophys. Acta 211:458–466Google Scholar
  17. Lauger, P., Benz, R., Start, G., Bamberg, E., Jordan, P.C., Fahr, A., and Brock, W. (1981) Quart. Rev. of Biophys. 14:513–598CrossRefGoogle Scholar
  18. Lelkes, P.I. and Tandeter, H.Y. (1982) Biochim. Biophys. Acta 716:410–418Google Scholar
  19. Markin, V.S. and Liberman, Y.A. (1973) Dohl. Akad. Nauk. SSSR 201:975–978Google Scholar
  20. Papahadjopoulos, D., Jacobson, K., Nir, S., and Isac, T. (1973) Biochim. Biophys. Acta 311:330–348Google Scholar
  21. Ralston, E., Hiemland, L.M., Klausner, R.D., Weinstein, J.N., and Blumenthal, R. (1981) Biochim. Biophys. Acta 649:133–141Google Scholar
  22. Sequaris, J.M. and Miller, I.R. (1984) Bioelectrochem. and Bioenerg. In press.Google Scholar
  23. Smith, R.M. and Martell, A.F. (1976) Critical Stability Constants. Plenum PressGoogle Scholar
  24. Urban, B.W., Hladky, S.B., and Haydon, D.A. (1980) Biochim. Biophys. Acta 602:331–354Google Scholar
  25. Wilshut, J. and Papahadjopoulos, D. (1979) Nature 282:690–692CrossRefGoogle Scholar
  26. Yager, P. and Peticolas, W.L. (1982) Biochim. Biophys. Acta 668:775Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Israel R. Miller
    • 1
  1. 1.Dept. of Membrane ResearchThe Weizmann Institute of Science RehovotRehovotIsrael

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