Abstract
The rate of relase of Tl+ from phospholipid vesicles of different composition was measured by pulse polarography as a function of temperature or in the presence of valinomycin, tetraphenyl boron (TPB‒) or dipicylamine (DPA‒) as transport facilitators. The release from pure dipalmitoyl phosphatidylcholine (DPPC) vesicles increased abruptly around the pretransition temp-earture. The steepness of the increase decreased with the width of the transition peak. Valinomycin TPB‒ (tetraphenyl boron) and DPA- (dipicryl amine) facilitate release of Tl+ from unilamellar vesicles above their phase transition temperature with a first order release rate constant. They do not facilitate release below the phase transition. Bursts of release were observed upon their addition to the vesicles but after annealing, which was completed within less than a minute, the vesicles were re-sealed. The rate of phase transitions, their reversibility or irreversibility and hysteresis with concomitant existence of metastable states, will be discussed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Benz, J., Duzgunes, N. and Nir, S. (1983) Biochemistry 22:3320–3330
Blok, M.C., van Deenen, L.M., and de Gier, J. (1976) Biochim. Biophys. Acta 433:1–12
Bunov, M.R. (1979) Biochim. Biophys. Acta 74:542–546
Ciani, S., Laprade, R., Eisenman, G., and Szabo, G. (1973) J. Memb. Biol. 11:255–292
Davis, P.J., Coolbear, K.P., and Keough, K.M.W. (1980) Can. J. Biochem. 54:851–859
Deamer, D.D. and Bangham, A.D. (1976) Biochim. Biophys. Acta 443:629–634
Eisenman, G., Kranse, S., and Ciani, S. (1975) Ann. NY Acad. Sci 264:34–60
Elamrani, K., and Blume, A. (1984) Biochemistry 22:3305–3311
Estep, T.N., Calhoun, W.I., Barneholz, Y., Bilton, R., Shipley, G.G., and Thompson, T.E. (1980) Biochemistry 19:20–24
Everett, D.H. (1978) Some biological implications of hysteresis in ions on macromolecular and biological systems, Ed. D.H. Everett and B. Vincent, Colston Papers no. 29, pp. 129–200
Freire, E., Bach, D., Correa-Freire, M., Miller, I.R., and Barenholz, Y., (1980) Biochemistry 19:3662–3665
Gutknecht, J. (1983) Biochim. Biophys. Acta 735:185–188
Hinz, H.J., and Sturtevan, J.M. (1972) J. Biol. Chem. 247:6071–6075
Hladky, S.B. (1979) The carrier mechanism in current topics. In: Membranes and Transport, 12:53–164. Eds: F. Bonner and A. Kleinzeller. Academic Press
Klein, R.A. (1982) Quart. Rev. of Biophys. 15:667–757
Lauger, P. and Stark, G. (1970) Biochim. Biophys. Acta 211:458–466
Lauger, P., Benz, R., Start, G., Bamberg, E., Jordan, P.C., Fahr, A., and Brock, W. (1981) Quart. Rev. of Biophys. 14:513–598
Lelkes, P.I. and Tandeter, H.Y. (1982) Biochim. Biophys. Acta 716:410–418
Markin, V.S. and Liberman, Y.A. (1973) Dohl. Akad. Nauk. SSSR 201:975–978
Papahadjopoulos, D., Jacobson, K., Nir, S., and Isac, T. (1973) Biochim. Biophys. Acta 311:330–348
Ralston, E., Hiemland, L.M., Klausner, R.D., Weinstein, J.N., and Blumenthal, R. (1981) Biochim. Biophys. Acta 649:133–141
Sequaris, J.M. and Miller, I.R. (1984) Bioelectrochem. and Bioenerg. In press.
Smith, R.M. and Martell, A.F. (1976) Critical Stability Constants. Plenum Press
Urban, B.W., Hladky, S.B., and Haydon, D.A. (1980) Biochim. Biophys. Acta 602:331–354
Wilshut, J. and Papahadjopoulos, D. (1979) Nature 282:690–692
Yager, P. and Peticolas, W.L. (1982) Biochim. Biophys. Acta 668:775
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Miller, I.R. (1986). Facilitation of Ion Permeability of Bilayer Membranes and their Phase Transition. In: Blank, M. (eds) Electrical Double Layers in Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8145-7_6
Download citation
DOI: https://doi.org/10.1007/978-1-4684-8145-7_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8147-1
Online ISBN: 978-1-4684-8145-7
eBook Packages: Springer Book Archive