Donnan Potentials Generated by the Surface Charge on Muscle Filaments
Electrochemical potentials (measured with microelec-trodes) in the liquid-crystalline lattices of striated muscle result from Donnan equilibriums established by the fixed charge associated with the myosin rods and actin filaments in the ionic cytosol. From these electrochemical potentials and the unit-cell volumes (measured by x-ray diffraction) together with Donnan theory, the effective linear charge densities are derived for the respective myosin rods and actin filaments. Electrochemical potentials also were measured under conditions that varied the parameters of the Donnan equation. Modeling predicted Donnan potentials for the same conditons. The predicted Donnan potentials correlate well with the measured electrochemical potentials for both the A-band and I-band liquid-crystalline lattices.
KeywordsActin Filament Electrical Double Layer Sarcomere Length Electrochemical Potential Fixed Charge
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