Electrochemical and Macromolecular Interactions at Red Blood Cell Surface
Red blood cells aggregate to form rouleaux in the presence of macro-molecules. Neutral and charged macromolecules were used to induce RBC aggregation quantified by microscopic observation. Variations of cell surface potential were achieved by the removal of RBC surface charge with neuraminidase treatment or by changing the ionic composition of the fluid medium. RBC aggregation by neutral polymer dextran is enhanced by removal of RBC surface charge and decreased by reduction of ionic strength. RBC aggregation by heparin requires the presence of sialic acids at cell surface and enhanced by reduction of ionic strength. It is concluded that the surface charge of RBCs plays a significant role in cell-to-cell interactions.
KeywordsIonic Strength Sialic Acid Intrinsic Viscosity Diffuse Double Layer Neuraminidase Treatment
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- Brooks, D. E., Charalambous, J., and Janzen, J., 1978, The molecular mechanism of erythrocyte aggregation, in: “Proc. Third Internat. Congr. on Biorheology”, Y. C. Fung and J. G. Pinto, ed.), LaJolla, CA., pp. 91.Google Scholar
- Chien, S., 1975, Biophysical Behavior of red cells in suspensions, in: “The Red Blood Cell”, 2nd ed.; D. MacN. Surgenor, ed., Academic Press New York; Vol. 2, pp. 1031.Google Scholar
- Fåhraeus, R., 1929, The suspension stability of blood. Physiol. Rev., 9:241.Google Scholar
- Houwink, R., 1952, The formation and structure of macromolecules. Macromolecular sols without electrolyte character, in: “Colloid Science”, H.R., Kruyt, ed., Elsevier Publishing Co., Amsterdam, Houston, New York, London, Vol. II, Chapter II and VI.Google Scholar
- Jan, K.-M., Usami, S., and Chien, S., 1982, The disaggregation effect of dextran 40 on red cell aggregation in macromolecular suspensions. Biorheol., 19:543.Google Scholar
- Jan, K.-M., 1985, Roles of surface electrochemistry and macromolecular adsorption in heparin-induced red blood cell aggregation. Biorheol., in press.Google Scholar
- LaMer, V. K., and Healy, T. W., 1963, Adsorption-flocculation reaction of macromolecules at the solid-liquid interface. Rev. Pure Appl. Chem. 13:112.Google Scholar
- Overbeek, J. Th. G., 1952, Electrochemistry of the double layer. Electrokinetic phenomena. Interaction between colloidal particles. Kinetics of flocculation, in: “Colloid Science”, H.R. Kruyt, ed., Elsevier Publishing Co., Amsterdam, Houston, New York, London, vol. II, Chap. IV–VI.Google Scholar
- Overbeek, J. Th. G., and Bungenberg de Jong, H. G., 1952, Sols of macromolecular colloids with electrolytic nature, in: “Colloid Science”, H.R. Kruyt, ed., Elsevier Publishing Co., Amsterdam, Houston, New York, London; vol. II, Chap. VIII.Google Scholar
- Ponder, E., 1948, “Hemolysis and related phenomena”, Grune and Stratton, New York, pp. 398.Google Scholar
- Tanford, C., 1961, “Physical chemistry of macromolecules”, John Wiley and Sons, Inc., New York, London Sidney, pp. 710.Google Scholar
- Thorsén, G., and Hint, J., 1950, Aggregation, sedimentation and intravascular sludging of erythrocytes. Acta Chir. Scand. (Suppl.), 154:1.Google Scholar