Effects of PH before and after Deep-Freeze Storage at -80°C on the Zeta Potentials of Bones

  • William K. Elwood
  • Stephan D. Smith
  • H. E. McKean


During the investigation of electroosmosis with bone in vitro (Elwood and Smith, 1983, 1984c), we became concerned about the effects of storage and storage media on the electrokinetic properties of bone. When changes in the electrokinetic characteristics of bone in vitro were monitored by measuring streaming potentials and calculating zeta(ζ)-poten-tials it was found that storage media could affect the ζ-potentials of bone stored overnight at 4°C or for several weeks at -80°C (Elwood and Smith 1984a, 1984b). Changes in the electrokinetic properties of the bone were considered to be reflective of its chemical and physical state, and hence of its possible suitability as a bone bank specimen. The changes were also considered to be a likely result of ion adsorption to or exchange between the bone surface and the “fixed” layer of electrolyte solution adjacent to its surface, which includes the double layer discussed in other contributions to this symposium.


Zeta Potential Trend Line Specific Conductivity Storage Medium Streaming Potential 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • William K. Elwood
    • 1
  • Stephan D. Smith
    • 1
  • H. E. McKean
    • 1
  1. 1.Departments of Anatomy and StatisticsUniversity of Kentucky A.B. Chandler Medical CenterLexingtonUSA

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