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Electrorotation of Single Cells — A New Method for Assessment of Membrane Properties

  • Roland Glaser
  • Günter Fuhr

Abstract

Cells placed in a rotating high-frequency field spin slowly at two characteristic frequencies fc1 and fc2 against and according to the rotatation of the field, respectively. This method, designated here as ELECTROROTATION, allows to measure membrane capacity, membrane conductivity and internal conductivity of single cells. Basic equations determining this process are given and correlated with experimental data. The dependence of electrorotation on external, membraneous, and internal conditions during the experiment is demonstrated. For practical use of this method it is sufficient to measure the first characteristic frequency (fc1), and additionally the frequency where the spin of the cells converts (f0). Measurements on plant protoplasts, erthrocytes and platelets indicate a lower relative membrane capacity as expected.

Keywords

Characteristic Frequency Membrane Conductivity Membrane Capacity Cell Radius Plant Protoplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Roland Glaser
    • 1
  • Günter Fuhr
    • 1
  1. 1.Department of BiologyHumboldt-Universität zu BerlinGermany

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