Electrorotation of Single Cells — A New Method for Assessment of Membrane Properties
Cells placed in a rotating high-frequency field spin slowly at two characteristic frequencies fc1 and fc2 against and according to the rotatation of the field, respectively. This method, designated here as ELECTROROTATION, allows to measure membrane capacity, membrane conductivity and internal conductivity of single cells. Basic equations determining this process are given and correlated with experimental data. The dependence of electrorotation on external, membraneous, and internal conditions during the experiment is demonstrated. For practical use of this method it is sufficient to measure the first characteristic frequency (fc1), and additionally the frequency where the spin of the cells converts (f0). Measurements on plant protoplasts, erthrocytes and platelets indicate a lower relative membrane capacity as expected.
KeywordsCharacteristic Frequency Membrane Conductivity Membrane Capacity Cell Radius Plant Protoplast
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