Transient Impedance Measurements on Biological Membranes: Application to Red Blood Cells and Melanoma Cells

  • R. Schmukler
  • J. J. Kaufman
  • P. C. Maccaro
  • J. T. Ryaby
  • A. A. Pilla


A technique has previously been described (1–5) by which the impedance of isolated cells can be evaluated over a wide frequency range (DC -20 MHz). The methodology centers about the use of a polycarbonate filter containing well defined and uniform cylindrical pores. A pseudo-epithelium is achieved by the entrapment of cells from a suspension in these pores using hydrostatic pressure. Data analysis by discrete Laplace transformation of the input and output waveforms allows electrochemical kinetic models to be tested via aperiodic equivalent circuit representation. In this study the effect of the state of the cell surface on the impedance parameters is examined. This is achieved by the use of the enzyme neuraminidase on the human red blood cell (HRBC) to reduce the surface charge and by melanocyte stimulating hormone (MSH) to alter the state of differentiation of melanoma cells.


Melanoma Cell Tyrosinase Activity Sialic Acid Residue Impedance Parameter Melanocyte Stimulate Hormone 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • R. Schmukler
    • 1
  • J. J. Kaufman
    • 1
  • P. C. Maccaro
    • 1
  • J. T. Ryaby
    • 1
  • A. A. Pilla
    • 1
  1. 1.Bioelectrochemistry Laboratory, Department of OrthopaedicsMount Sinai School of MedicineNew YorkUSA

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