Genetic and Structural Studies of a V-Region Marker in Mouse Immunoglobulin Light Chains

  • Paul D. Gottlieb


This chapter describes the characteristics and strain distribution of a genetic marker discovered by Edelman and Gottlieb (1970) in the V regions of serum Ig L chains of unimmunized mice. Gottlieb (1974) demonstrated that expression of this V-region marker (called the IB-peptide marker) is closely linked to the Ly-3 genetic locus governing expression of a thymocyte surface antigen (Boyse et al., 1971). The Ly-3 locus resides in linkage group XI on chromosome 6 of the mouse (Itakura et al., 1972). If the locus governing expression of the IB-peptide marker is a structural locus for L-chain V regions, then it too resides in linkage group XI, and this would be the first instance in any species in which structural genes for an Ig chain have been placed in a particular linkage group. The possibility exists, however, that the locus which maps in linkage group XI does not contain structural genes for light-chain V regions, but rather is a regulatory locus which governs the expression of structural genes encoded in this linkage group or elsewhere in the genome.


Light Chain Inbred Strain Iodoacetic Acid Unimmunized Mouse Multiple Amino Acid Substitution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baltimore, D., 1914,Nature (London) 248:409.CrossRefGoogle Scholar
  2. Barstad, P., Rudikoff, S., Potter, M., Cohn, M., Konigsberg, W., and Hood, L., 1974, Science 183:962.PubMedCrossRefGoogle Scholar
  3. Blinz, H., and Wigzell, H., 1975, J. Exp. Med. 142:197.CrossRefGoogle Scholar
  4. Blomberg, B., Geckeler, W., and Weigert, M., 1972, Science 177:178.PubMedCrossRefGoogle Scholar
  5. Bosma, M., and Bosma, G., 1974, J. Exp. Med. 139:512.PubMedCrossRefGoogle Scholar
  6. Boyse, E.A., Miyazawa, M., Aoki, T., and Old, L.J., 1968a, Proc. Roy. Soc. London Ser. B 170:175.CrossRefGoogle Scholar
  7. Boyse, E.A., Old, L.J., and Stockert, E., 1968b, Proc. Natl. Acad. Sci. USA 60:886.PubMedCrossRefGoogle Scholar
  8. Boyse, E.A., Itakura, K., Stockert, E., Iritani, C.A., and Miura, M., 1971, Transplantation 11:351.PubMedCrossRefGoogle Scholar
  9. Brenner, S., and Milstein, C, 1969, Nature (London) 211:242.CrossRefGoogle Scholar
  10. Cantor, H., and Boyse, E.A., 1975a, J. Exp. Med. 141:1376.PubMedCrossRefGoogle Scholar
  11. Cantor, H., and Boyse, E.A., 1975b, J. Exp. Med. 141:1390.PubMedCrossRefGoogle Scholar
  12. Cantor, H., and Simpson, E., 1975, Eur. J. Immunol. 5:330.PubMedCrossRefGoogle Scholar
  13. Claflin, J.L., and Davie, J.M., 1975, J. Immunol. 114:70.PubMedGoogle Scholar
  14. Cohn, M., Blomberg, B., Geckeler, W., Raschke, W., Riblet, R., and Weigert, M., 1974, in: The Immune System, Genes, Receptors, Signals (E.E. Sercarz, A.R. Williamson, and C.F. Fox, eds.), p. 89, Academic Press, New York.Google Scholar
  15. Cunningham, B.A., Gottlieb, P.D., Pflumm, M.N., and Edelman, G.M., 1971, in: Progress in Immunology (B. Amos, ed.), pp. 3–24, Academic Press, New York.Google Scholar
  16. David, G.S., and Todd, C.W., 1969, Proc. Natl. Acad. Sci. USA 62:860.PubMedCrossRefGoogle Scholar
  17. Dray, S., and Nisonoff, A., 1963, Proc. Soc. Exp. Biol. Med. 113:20.Google Scholar
  18. Dray, S., Kim, B.S., and Gilman-Sachs, A., 1914, Ann. Immunol. 125:41.Google Scholar
  19. Dreyer, W., Gray, W.R., and Hood, L., 1967, Cold Spring Harbor Symp. Quant. Biol. 32:353.CrossRefGoogle Scholar
  20. Dugan, E.S., Bradshaw, R.A., Simms, E.S., and Eisen, H.N., 1973.Biochemistry 12:5400.PubMedGoogle Scholar
  21. Durda, P.J., and Gottlieb, P.D., 1976, J. Exp. Med. 144:476.PubMedCrossRefGoogle Scholar
  22. Edelman, G.M., and Gottlieb, P.D., 1970, Proc. Natl. Acad. Sci. USA 67:1192.PubMedCrossRefGoogle Scholar
  23. Eichmann, K., 1973, J. Exp. Med. 137:603.PubMedCrossRefGoogle Scholar
  24. Eichmann, K., and Rajewsky, K., 1975, Eur. J. Immunol. 5:661.PubMedCrossRefGoogle Scholar
  25. Fahey, J.L., Carbone, P.O., Rowe, D.S., and Bachmann, R., 1968, Am. J. Med. 45:373.PubMedCrossRefGoogle Scholar
  26. Fleischman, J.B., 1911, Biochemistry 10:2753.CrossRefGoogle Scholar
  27. Gaily, J.A., 1973, in: The Antigens, Vol. I (M. Sela, ed.), p. 161, Academic Press, New York.Google Scholar
  28. Gaily, J.A., and Edelman, G.M., 1970, Nature (London) 227:341.CrossRefGoogle Scholar
  29. Gaily, J.A., and Edelman, G.M., 1972, Annu. Rev. Genet. 6:1.CrossRefGoogle Scholar
  30. Gasser, D.L., 1969, J. Immunol. 103:66.PubMedGoogle Scholar
  31. Gottlieb, P.D., 1971, Ph.D. thesis, Rockefeller University.Google Scholar
  32. Gottlieb, P.D., 1974, J. Exp. Med. 140:1432.PubMedCrossRefGoogle Scholar
  33. Green, M.C., and Schultz, L., 1975, J Hered. 66:250.PubMedGoogle Scholar
  34. Gutman, G.A., Loh, E., and Hood, L., 1975, Proc. Natl Acad. Sci. USA 72:5046.PubMedCrossRefGoogle Scholar
  35. Itakura, K., Hutton, J.J., Boyse, E.A., and Old, L.J., 1971, Nature (London) New Biol. 230:126.CrossRefGoogle Scholar
  36. Itakura, K., Hutton, J.J., Boyse, E.A., and Old, L.J., 1972, Transplantation 13:239.PubMedCrossRefGoogle Scholar
  37. Jaton, J.C., and Braun, D.E., 1972,Biochem. J. 130:539.PubMedGoogle Scholar
  38. Kindt, T.J., 1975, in: Advances in Immunology, Vol. 21 (F.J. Dixon, and H.G. Kunkel, eds.), p. 35, Academic Press, New York.CrossRefGoogle Scholar
  39. Kindt, T.J., Todd, C.W., Eichmann, K., and Krause, R.M., 1970, J. Exp. Med. 131:343.PubMedCrossRefGoogle Scholar
  40. Kisielow, P., Hirst, J.A., Beverley, P.C.L., Hoffman, M.K., Boyse, E.A., and Oettgen, H.F., 1975, Nature (London) 253:219.CrossRefGoogle Scholar
  41. Klein, J., 1973, Transplantation (Baltimore) 15:137.CrossRefGoogle Scholar
  42. Klein, J., 1975, Biology of the Mouse Histocompatibility-2 Complex, Springer-Verlag, New York.CrossRefGoogle Scholar
  43. Komuro, K., Itakura, K., Boyse, E.A., and John, M., 1915,Immunogenetics 1:452.CrossRefGoogle Scholar
  44. Landucci-Tosi, S., Tosi, R., and Perramon, A., 1973, J. Immunol. 110:286.Google Scholar
  45. Leder, P., Honjo, T., Swan, D., Packman, S., Nau, M., and Norman, B., 1975, in: MolecularApproaches to Immunology, Vol. 9 (E.E. Smith and D.W. Robbins, eds.), pp. 173–188, Miami Winter Symposia, Academic Press, New York.CrossRefGoogle Scholar
  46. Lieberman, R., Potter, M., Mushinski, E.B., Humphrey, W., and Rudikoff, S., 1974, J. Exp. Med. 139:983.PubMedCrossRefGoogle Scholar
  47. Mage, R., Lieberman, R., Potter, M., and Terry, W.D., 1973, in: The Antigens, Vol. I (M. Sela, ed.), p. 299, Academic Press, New York.Google Scholar
  48. Mole, L.E., Jackson, S.A., Porter, R.R., and Wilkinson, J.M., 1911,Biochem. J. 124:301.Google Scholar
  49. Mudgett, M., Fraser, B.A., and Kindt, T.J., 1975, J. Exp. Med. 141:1448.PubMedCrossRefGoogle Scholar
  50. Pawlak, L.L., Mushinski, E.B., Nisonoff, A., and Potter, M., 1973, J. Exp. Med. 137:22.PubMedCrossRefGoogle Scholar
  51. Porter, R.R., 1974, Ann. Immunol (Inst. Pasteur) 125C:85.Google Scholar
  52. Pothier, L., Borel, H., and Adams, R.A., 1974, J. Immunol. 113:1984.PubMedGoogle Scholar
  53. Potter, M., and Lieberman, R., 1970, J. Exp. Med. 132:737.PubMedCrossRefGoogle Scholar
  54. Prahl, J.W., Tack, B.F., and Todd, C.W., 1973, Biochemistry 12:5181.PubMedCrossRefGoogle Scholar
  55. Rivat, L., Gilbert, D., and Ropartz, C, 1973, Immunology 24:1041.PubMedGoogle Scholar
  56. Schultz, L.D., and Green, M.C., 1976, J. Immunol. 116:936.Google Scholar
  57. Shen, F.-W., Boyse, E.A., and Cantor, H., 1915, Immunogenetics 2:591.CrossRefGoogle Scholar
  58. Shiku, H., Kisielow, P., Bean, M.A., Takahashi, T., Boyse, E.A., Oettgen, H.F., and Old, L.J., 1915,J. Exp. Med. 141:227.CrossRefGoogle Scholar
  59. Shirai, T., and Mellors, R.C., 1971, Proc. Natl. Acad. Sci. USA 68:1412.PubMedCrossRefGoogle Scholar
  60. Shreffler, D.C., and David, C.S., 1975, Adv. Immunol. 20:125.PubMedCrossRefGoogle Scholar
  61. Simpson, E. and Cantor, H., 1915, Eur. J. Immunol. 5:337.CrossRefGoogle Scholar
  62. Škavřil, F., Brummelová, V. and Franěk, F., 1967, Biochim. Biophys. Acta 140:371.CrossRefGoogle Scholar
  63. Smithies, O., 1967, Science 157:267.PubMedCrossRefGoogle Scholar
  64. Smithies, O., 1970, Science 169:882.PubMedCrossRefGoogle Scholar
  65. Solomon, A., 1976, N. Engl. J. Med. 294:17.PubMedCrossRefGoogle Scholar
  66. Spring, S.B., and Nisonoff, A., 1974, J. Immunol. 113:470.Google Scholar
  67. Staats, J., 1972, Cancer Res. 32:1609.PubMedGoogle Scholar
  68. Stemke, G.W., 1965, Immunochemistry 25:359.CrossRefGoogle Scholar
  69. Strosberg, A.D., Hamers-Casterman, C, Van der Loo, W., and Hamers, R., 1974, J. Immunol. 113:1313.PubMedGoogle Scholar
  70. Tack, B.F., Feintuch, K., Todd, C.W., and Prahl, J.W., 1973, Biochemistry 12:5172.PubMedCrossRefGoogle Scholar
  71. Takasugi, M., and Hildemann, W.H., 1969, J. Natl Cancer Inst. 43:843.PubMedGoogle Scholar
  72. Tonegawa, S., 1976, Proc. Natl. Acad. Sci USA 73:203.PubMedCrossRefGoogle Scholar
  73. Vice, J.L., Gilman-Sachs, A., Hunt, W.L., and Dray, S., 1970, J. Immunol. 104:550.PubMedGoogle Scholar
  74. Waterfield, M.D., Prahl, J.W., Hood, L.E., Kindt, T.J., and Krause, R.M., 1972, Nature (London) New Biol. 240:215.CrossRefGoogle Scholar
  75. Weigert, M., Cesari, I.M., Yonkovich, S.J., and Conn, M., 1970, Nature (London) 228:1045.CrossRefGoogle Scholar
  76. Weigert, M., Potter, M., and Sachs, D., 1975, Immunogenetics 1:511.CrossRefGoogle Scholar
  77. Wilkinson, J.M., 1969a, Biochem. J. 112:173.PubMedGoogle Scholar
  78. Wilkinson, J.M., 1969b, Nature (London) 223:616.CrossRefGoogle Scholar
  79. Williams, C.A., and Chase, M.W., eds., 1967, in: Methods in Immunology and Immunochemistry, Vol. I, Academic Press, New York.Google Scholar
  80. Wu, T.T., and Kabat, E.A., 1970, J. Exp. Med. 132:211.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Paul D. Gottlieb
    • 1
  1. 1.Department of Biology and Center for Cancer ResearchMassachusetts Institute of TechnologyCambridgeUSA

Personalised recommendations