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The Effect of High Intensity U.V. Radiation on Benthic Marine Algae

  • M. Polne
  • A. Gibor
Part of the NATO Conference Series book series (NATOCS, volume 7)

Abstract

We are using fluorescence microscopy in the determination of damage caused to algal cells by various chemical and physical treatments. Damage is manifest as a change in the fluorescence spectrum of the cells. Fluorescence microscopy exposes cells to short-blue. (400–460 nm) and UV-A (315–400 nm) light. Shorter wavelengths in the UV-B range are eliminated by the glass microscope lenses and filters. We observed that exposure of algal tissue to the exciting beam of the fluorescence microscope also causes damage to cells which can be seen as a change in the fluorescence spectrum over time during exposure. We have attempted to determine the degree of damage to the algae as a result of this exposure and to describe the progress of the damage to the cells.

Keywords

Fluorescence Spectrum Cence Microscope Algal Pigment Algal Tissue Exciting Beam 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Abeliovich, A. and M. Shilo. 1972. Photooxidative death in blue-green algae, J. Bact., Vol. III (3), 682–689.Google Scholar
  2. Bener, P. 1972. Approximate values intensity of natural ultraviolet radiation for different amount of atmospheric ozone. European Research Office, US Army, London. Contract number DAJA 37–68-C-1017, 4–59.Google Scholar
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  4. Stair, R. 1969. Measurements of natural ultraviolet radiation, historical and general introduction. In: The Biologic Effect of Ultraviolet Radiation. [F. Urbach ed.] Pergamon Press, Oxford.Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • M. Polne
    • 1
  • A. Gibor
    • 1
  1. 1.Biological Sciences and Marine Science InstituteUniversity of CaliforniaSanta BarbaraUSA

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