Some Topological and Dynamic Aspects of Lipids in the Erythrocyte Membrane
This paper reviews the use of phospholipases to determine the topology of phospholipids in the erythrocyte membrane. In mammalian red cell membranes the choline-containing phospholipids, sphingomyelin and lecithin are predominantly located at the exterior region whereas phosphatidylethanolamine and phosphatidylserine are found preferentially in the inner monolayer. A topological asymmetry of phospholipid metabolism was detected in rat erythrocyte membranes. Exchange of phospholipids between plasma lipoproteins and intact red cells occured at the exterior region of the lipid bi-layer. The incorporation of fatty acids into phospholipids appeared to be located at the interior side of the membrane. The data indicate that a translocation of lecithin species between the two pools or sides of the membrane may be possible.
Electron microscopic studies demonstrated that the enlargement of erythrocytes in patients with cholestasis is due to a fusion of abnormal plasma lipoprotein with the red cell membrane, resulting in an increased content of lecithin and cholesterol.
KeywordsErythrocyte Membrane Fracture Face Phospholipase Action Human Erythrocyte Membrane Intact Erythrocyte
Unable to display preview. Download preview PDF.
- Cooper, R.A., Lipids of human red cell membrane: Normal composition and variability in disease, Semin. Hematol., 7, 296, 1970.Google Scholar
- van Deenen, L.L.M., J. de Gier, Lipids of the red cell membrane, in The Red Blood Cell, edited by D. Surgenor, pp. 147–211, Academic Press, New York, 1974.Google Scholar
- Kahlenberg, A., C. Walker aFR. Rohrlick, Evidence for an asymme-tric distribution of phospholipids in the human erythrocyte membrane, Can. J. Biochem., 52, 803, 1974.Google Scholar
- Verkleij, A.J., R.F.A. Zwaal, B. Roelofsen, P. Comfurius, D. Kastelijn and L.L.M. van Deenen, The asymmetric distribution of phospholipids in the human red cell membrane. A combined study using phospholipases and freeze-etch electron microscopy, Biochim. Biophys. Acta, 323, 178, 1973.CrossRefGoogle Scholar
- Verkleij, A.J., I.L.D. Nauta, J.M. Werre, J.G. Mandersloot, B. Reinders, P.H.J.Th. Ververgaert and J. de Gier, The fusion of abnormal plasma lipoprotein (LP-X) and the erythrocyte membrane in patients with cholestasis studied by electron microscopy, Biochim. Biophys. Acta, 1976, in the press.Google Scholar
- Zwaal, R.F.A., B. Roelofsen and C.M. Colley, Localization of red cell membrane constituents, Biochim. Biophys. Acta, 300, 159, 1973.Google Scholar
- Zwaal, R.F.A., B. Roelofsen, P. Comfurius and L.L.M. van Deenen, Organisation of phospholipids in human red cell membranes as detected by the action of various purified phospholipases, Biochim. Biophys. Acta, 406, 83, 1975.Google Scholar