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Some Topological and Dynamic Aspects of Lipids in the Erythrocyte Membrane

  • L. L. M. van Deenen
  • J. de Gier
  • L. M. G. van Golde
  • I. L. D. Nauta
  • W. Renooy
  • A. J. Verkleij
  • R. F. A. Zwaal
Part of the Nobel Foundation Symposia book series (NOFS, volume 34)

Abstract

This paper reviews the use of phospholipases to determine the topology of phospholipids in the erythrocyte membrane. In mammalian red cell membranes the choline-containing phospholipids, sphingomyelin and lecithin are predominantly located at the exterior region whereas phosphatidylethanolamine and phosphatidylserine are found preferentially in the inner monolayer. A topological asymmetry of phospholipid metabolism was detected in rat erythrocyte membranes. Exchange of phospholipids between plasma lipoproteins and intact red cells occured at the exterior region of the lipid bi-layer. The incorporation of fatty acids into phospholipids appeared to be located at the interior side of the membrane. The data indicate that a translocation of lecithin species between the two pools or sides of the membrane may be possible.

Electron microscopic studies demonstrated that the enlargement of erythrocytes in patients with cholestasis is due to a fusion of abnormal plasma lipoprotein with the red cell membrane, resulting in an increased content of lecithin and cholesterol.

Keywords

Erythrocyte Membrane Fracture Face Phospholipase Action Human Erythrocyte Membrane Intact Erythrocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • L. L. M. van Deenen
    • 1
  • J. de Gier
    • 1
  • L. M. G. van Golde
    • 1
  • I. L. D. Nauta
    • 1
  • W. Renooy
    • 1
  • A. J. Verkleij
    • 1
  • R. F. A. Zwaal
    • 1
  1. 1.Laboratory of Biochemistry and Laboratory of Veterinary BiochemistryUniversity of UtrechtThe Netherlands

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