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Proton Translocation by Bacteriorhodopsin in Model Systems

  • Walther Stoeckenius
  • San-Bao Hwang
  • Juan Korenbrot
Part of the Nobel Foundation Symposia book series (NOFS, volume 34)

Abstract

Bacteriorhodopsin is a rhodopsin-like pigment found in the cell membrane of halobacteria. It occurs in discrete patches with a planar hexagonal lattice structure known as the purple membrane (Oesterhelt and Stoeckenius, 1971; Blaurock and Stoeckenius, 1971). When bacteriorhodopsin absorbs light, it undergoes a rapid cyclic photoreaction during which it translocates a proton across the membrane (Lozier et al, 1975; Lozier et al, 1976). In continuous light it acts as a light-driven proton pump generating a proton gradient and membrane potential. The cells can use the energy stored in the electrochemical gradient to synthesize ATP (Oester-helt and Stoeckenius, 1973; Bogomolni et al, 1976; Danon and Stoeckenius, 1974). In intact cells it is difficult to measure parameters such as rapid absorption changes of the pigment, intracellular ion concentrations, and membrane potential, which are necessary to quantitate the light energy conversion in this system. Moreover, the energy metabolism of halobacteria has not been investigated in detail and contributions from other energy sources are difficult to evaluate. Reconstitution of the bacteriorhodopsin function in a well-characterized model system avoids most of the difficulties encountered in work with intact cells.

Keywords

Lipid Vesicle Proton Acceptor Planar Film Fracture Face Soybean Lecithin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Walther Stoeckenius
    • 1
  • San-Bao Hwang
    • 1
  • Juan Korenbrot
    • 1
  1. 1.Cardiovascular Research InstituteUniversity of California, San FranciscoSan FranciscoCanada

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