X-ray Scattering Studies of Protein-Lipid Systems in Solution: The Example of Bovine Rhodopsin

  • Annette Tardieu
  • Christian Sardet
  • Vittorio Luzzati
Part of the Nobel Foundation Symposia book series (NOFS, volume 34)


Small-angle X-ray scattering, one of the most powerful techniques for the study of macromolecules in solution, has been applied to a variety of systems of biological interest. A shortcoming of this technique, especially if compared to a conventional crystallographic approach, is its poor resolution, orders of magnitude short of atomic resolution; its main advantage is the possibility of easily varying physical and chemical conditions and exploring structural transitions. Furthermore, biological systems are not always amenable to high resolution crystallographic analysis.


Bovine Rhodopsin Partial Specific Volume Detergent Micelle Rhodopsin Molecule Electron Density Fluctuation 
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  1. Applebury, M.L., Zuckerman, D.M. Lamola, A.A. and Jovin, T.M. (1974), Biochem., 13, 3448–3458.Google Scholar
  2. Daemen, F.J.M., De Grip, W.J. and Jansen, P.A.A. (1972), Biochim. Biophys. Acta, 271, 419–428.Google Scholar
  3. Ebrey, T.G. (1971), Vision Res.,ll, 1007–1009.Google Scholar
  4. Gabriel, A. and Dupont, Y. ( 1972, Rev. Sci. Instrum., 112, 1600–1603.CrossRefGoogle Scholar
  5. Helenius, A. and Simons, K. (1975), Biochim. Biophys. Acta, 415, 29–79.Google Scholar
  6. Henderson, R. et Unwin, P.N.T. (1975), Nature, 257, 28–32.PubMedCrossRefGoogle Scholar
  7. Hong, K., Hubbell, W.L. (1973), Biochemistry, 12, 4517–4523.PubMedCrossRefGoogle Scholar
  8. Luzzati, V. (1960), Acta Cryst., 13, 939–945.CrossRefGoogle Scholar
  9. Luzzati, V., Tardieu, A., Mateu, L. and Stuhrmann, H.B. (1976), J. Mol. Biol., 101, 115–127.PubMedCrossRefGoogle Scholar
  10. Luzzati, V., Witz, J. and Baro, R. (1963), Journal de Physique, 24, 141–1Ä6A.Google Scholar
  11. Luzzati, V., Witz, J. and Nicolaieff, A. (1961), J. Mol. Biol., 3, 367–378.CrossRefGoogle Scholar
  12. Osborne, B.H., Sardet, C. and Helenius, A. (1974), Eur. J. Biochem., 44, 383–390.PubMedCrossRefGoogle Scholar
  13. Pober, J. and Stryer, L. (1975), J. Mol. Biol., 95, 477–481.Google Scholar
  14. Robinson, N.C. and Tanford, C. (1975), Biochem., 14, 369–378.CrossRefGoogle Scholar
  15. Sardet, C., Tardieu, A. and Luzzati, V. (1976), J. Mol. Biol., in press.Google Scholar
  16. Schmatz, W., Springer, T. Schelten, J. and Ibel, K. (1974), J. Appl. Cryst., 7, 96–116.CrossRefGoogle Scholar
  17. Stubbs, G.W., Smith, H.G. and Litman, B.J. (1976), Biochim. Biophys. Acta, 425, 46–56.Google Scholar
  18. Stuhrmann, H.B. and Kirste, R.G. (1967), Z. Physik. Chemie, Nune Folge, 56 334–337.CrossRefGoogle Scholar
  19. Stuhrmann, H.B., Tardieu, A., Mateu, L., Sardet, C., Luzzati, V., Aggerbeck, L. and Scanu, A.M. (1975), Proc. Nat. Acad. Sei. USA, 72, 2270–2273.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Annette Tardieu
    • 1
  • Christian Sardet
    • 1
  • Vittorio Luzzati
    • 1
  1. 1.Centre de Génétique Moléculaire du C.N.R.SGif-sur-YvetteFrance

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