Phospholipid Metabolism in Some Excitable Biological Membranes

  • J. N. Hawthorne
Part of the Nobel Foundation Symposia book series (NOFS, volume 34)

Abstract

Triphosphoinositide, a constituent of myelin and plasma membranes, may be important in binding calcium ions. The purification to homogeneity of a phosphatase from brain which removes the 4- and 5-phosphate groups of this phospholipid is described. In iris muscle, acetylcholine activates the breakdown of triphosphoinositide, the effect being blocked by atropine but not tubocurarine. The related lipid phosphatidylinositol was most highly labelled in vivo in the membrane of transmitter vesicles from brain synaptosomes. Electrical stimulation of synaptosomes caused rapid breakdown of this phosphatidylinositol and of phosphatidic acid in another sub-synaptosomal membrane fraction. Possible functions of these lipids in transmitter release are discussed.

Keywords

Hydrolysis Cyanide Catecholamine Acetylcholine Ghost 

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References

  1. Abdel-Latif, A.A. and R. A. Akhtar, Acetylcholine causes an increase in the hydrolysis of triphosphoinositide pre-labelled with [32P]phosphate or [33H]inositol and a corresponding increase in the labelling of phosphatidylinositol and phosphatidic acid in rabbit iris muscle, Biochem. Soc. Trans. 4, 317, 1976.Google Scholar
  2. Allan, D. and R. H. Michell, Elevation of intracellular calcium ion concentration provokes production of 1,2-diacylglycerol and phosphatidate in human erythrocytes, Biochem. Soc. Trans., 3, 751, 1975.Google Scholar
  3. Birnberger, A.C., K. L. Birnberger, S. G. Eliasson and P. C. Simpson, Effect of cyanide and electrical stimulation on phosphoinositide metabolism in lobster nerves, J. Neurochem. 18, 1291, 1971.PubMedCrossRefGoogle Scholar
  4. Bleasdale, J.E. and J. N. Hawthorne, The effect of electrical stimulation on the turnover of phosphatidic acid in synaptosomes from guinea-pig brain, J. Neurochem. 24, 373, 1975.PubMedCrossRefGoogle Scholar
  5. Buckley, J.T. and J. N. Hawthorne, Erythrocyte membrane polyphospho- inositide metabolism and the regulation of calcium binding, J. Biol. Chem. 247, 7218, 1972.PubMedGoogle Scholar
  6. Cooper, P.H. and J. N. Hawthorne, Phosphomonoesterase hydrolysis of polyphosphoinositides in rat kidney, Biochem. J. 150, 537, 1975.PubMedGoogle Scholar
  7. Cotman, C.W., R. E. McCaman and S. A. Dewhurst, Subsynaptosomal distribution of enzymes involved in the metabolism of lipids, Biochim. Biophys. Acta, 249, 395, 1971.CrossRefGoogle Scholar
  8. Daleo, G.R., M. M. Piras and R. Piras, The presence of phospholipids and diglyceride kinase activity in microtubules from different tissues, Biochem. Biophys. Res. Commun. 61, 1043, 1974.CrossRefGoogle Scholar
  9. Dawson, R.M.C., Labelling of brain phospholipids with radioactive phosphorus, Biochem. J. 57, 237, 1954.PubMedGoogle Scholar
  10. De Belleroche, J.S. and H. F. Bradford, Metabolism of beds of mammalian cortical synaptosomes: response to depolarizing influences, J. Neurochem. 19, 585, 1972.PubMedCrossRefGoogle Scholar
  11. Garrett, N.E., R. J. B. Garrett, R. T. Talwalkar and R. L. Lester, Rapid breakdown of diphosphoinositide and triphosphoinositide in erythrocyte membranes, J. Cell.Physiol. 87, 63, 1975.CrossRefGoogle Scholar
  12. Hawthorne, J.N. and J. E. Bleasdale, Phosphatidic acid metabolism, calcium ions and transmitter release from electrically stimulated synaptosomes, Molec. Cell. Biochem. 8, 83, 1975.CrossRefGoogle Scholar
  13. Hawthorne, J.N. and P. Kemp, The brain phosphoinositides, Advan. Lipid Res. 2, 127, 1964.Google Scholar
  14. Hokin, M.R. and L. E. Hokin, Interconversions of phosphatidylinositol and phosphatidic acid involved in the response to acetylcholine in the salt gland, in Metabolism and Physiological Significance of Lipids (ed. R. M. C. Dawson and D. N. Rhodes ) p. 423, John Wiley, London, 1964.Google Scholar
  15. Lapetina, E.G. and J. N. Hawthorne, The diglyceride kinase of rat cerebral cortex, Biochem. J. 122, 171, 1971.PubMedGoogle Scholar
  16. Lunt, G.C. and M. R. Pickard, The subcellular localization of carbamylcholine-stimulated phosphatidylinositol turnover in rat cerebral cortex in vivo, J. Neurochem. 24, 1203, 1975.PubMedCrossRefGoogle Scholar
  17. Michell, R.H., Inositol phospholipids and cell surface receptor function, Biochim. Biophys. Acta, 415, 81, 1975.Google Scholar
  18. Salway, J.G. and I. E. Hughes, The possible role of phospho-inositides as regulators of action potentials: effect of electrical stimulation, tetrodotoxin and cinchocaine on phosphoinositide labelling by 32P in rabbit vagus, J. Neurochem. 19, 1233, 1972.PubMedCrossRefGoogle Scholar
  19. Schacht, J. and B. W. Agranoff, Effects of acetylcholine on labelling of phosphatidate and phosphoinositides by [32P]-orthophosphate in nerve ending fractions of guinea pig cortex, J. Biol. Chem. 247, 771, 1972.PubMedGoogle Scholar
  20. White, G.L., H. U. Schellhase and J. N. Hawthorne, Phosphoinositide metabolism in rat superior cervical ganglion, vagus and phrenic nerve: effects of electrical stimulation and various blocking agents, J. Neurochem. 22, 149, 1974.PubMedCrossRefGoogle Scholar
  21. Whittaker, V.P., I. A. Michaelson and R. J. A. Kirkland, The separation of synaptic vesicles from nerve-ending particles (’synaptosomes’), Biochem. J. 90, 293, 1964.PubMedGoogle Scholar
  22. Yagihara, Y., J. E. Bleasdale and J. N. Hawthorne, Effects of acetylcholine on the incorporation of [32P]orthophosphate in vitro into the phospholipids of subsynaptosomal membranes from guinea-pig brain, J. Neurochem. 21, 173, 1973.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • J. N. Hawthorne
    • 1
  1. 1.Department of BiochemistryUniversity Hospital and Medical SchoolNottinghamUK

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