Effect of Electrical and Chemical Stimulation on Protein Synthesis in Brain Slices

  • Fritz Lipmann


It is only natural that with our long-standing interest in protein synthesis(1) the recent lively discussion of the role of protein turnover in nerve function made it attractive to have another look at protein synthesis in the brain. Many years ago I worked for a while with pigeon brain slices in order to study the metabolic function of thiamine.(2) These experiments left me with the impression that, metabolically speaking, the brain slice gives a good reflection of in vivo events. It gave me special confidence that after cutting and exposure to low temperature the slices seemed slightly shocked because, during the first hour, an aerobic glycolysis appeared which, however, disappeared again on further incubation. In other words, the slice normalized during incubation at 37°; it could be used for up to 3 hr without showing any signs of decay.


Electrical Stimulation Brain Slice Potassium Chloride Aerobic Glycolysis Spreading Depression 
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  1. 1.
    F. Lipmann, Science 164, 1024–1031 (1969); F. Lipmann, in: “Metabolism of the Nervous System” (D. Richter, ed.), pp. 329–340, Pergamon Press, London (1957).Google Scholar
  2. 2.
    F. Lipmann, Scand. Arch. Physiol. 76, 255–272 (1937).Google Scholar
  3. 3.
    H. Mcllwain and R. Rodnight, “Practical Neurochemistry,” Little, Brown and Company, Boston (1959).Google Scholar
  4. 4.
    F. Orrego and F. Lipmann, J. Biol. Chem. 242, 665–671 (1967).PubMedGoogle Scholar
  5. 5.
    F. Orrego, J. Neurochem. 14, 851–858 (1967).CrossRefGoogle Scholar
  6. 6.
    H. Mcllwain, in: “Neurochemistry,” ( K. A. C. Elliott, I. H. Page, and J. H. Quastel, eds.), p. 212, Charles C. Thomas, Springfield, Ill. (1962).Google Scholar
  7. 7.
    L. C. Mokrasch and P. Manner, J. Neurochem. 10, 541–547 (1963).PubMedCrossRefGoogle Scholar
  8. 8.
    D. R. Curtis and J. C. Watkins, J. Physiol. 166 1–14 (1963).PubMedGoogle Scholar
  9. 9.
    P. J. Heald, “Phosphorus Metabolism of the Brain,” Pergamon, New York (1960).Google Scholar
  10. 10.
    G. S. Bennett and G. M. Edelman, Science, 163, 393–395 (1969).PubMedCrossRefGoogle Scholar
  11. 11.
    W. H. Marshall, Physiol. Rev. 39, 239–279 (1959).PubMedGoogle Scholar
  12. 12.
    S. Ochs. Int. Rev. Neurobiol. 4, 1 (1962).Google Scholar
  13. 13.
    M. Ruscak, J. Neurochem. 7, 305–307 (1961).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • Fritz Lipmann
    • 1
  1. 1.The Rockefeller UniversityNew YorkUSA

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