The Expression of the Gene for the Large Subunit of Ribulose 1,5-Bisphosphate Carboxylase in Maize

  • Gerhard Link
  • John R. Bedbrook
  • Lawrence Bogorad
  • Donald M. Coen
  • Alexander Rich
Part of the Basic Life Sciences book series (BLSC, volume 11)


During the past few years it has been established by DNA-RNA hybridization and physical mapping that the circular 85 × 106-dalton chloroplast chromosome of Zea mays contains genes for tRNAs for at least 16 amino acids (1) and two sets of genes for rRNAs (2). The chloroplast gene for ribosomal LC4 protein (3, 4) has been located on the Chlamydomonas reinhardi chloroplast chromosome by transmission genetics (5). We have now located physically the site of the gene for the large subunit (L) of the enzyme ribulose 1,5-bisphosphate (RuBP) carboxylase on the maize chloroplast genome. This has been possible because of progress in the physical mapping of this genome (6) and the availability of chloroplast (et) DNA sequences cloned in bacterial plasmids as well as the development of linked transcription-translation systems (7).


Large Subunit Bundle Sheath Bundle Sheath Cell Rabbit Reticulocyte Lysate Filtrate Residue 


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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Gerhard Link
    • 1
  • John R. Bedbrook
    • 1
  • Lawrence Bogorad
    • 1
  • Donald M. Coen
    • 2
  • Alexander Rich
    • 2
  1. 1.The Biological LaboratoriesHarvard UniversityCambridgeUSA
  2. 2.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA

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