Neutrophil Migration and Orientation Under Agarose: Findings in Patients with the Immotile Cilia Syndrome and Effects of Cytochalasin B and Vinblastine
The technique measuring the migration of PMN neutrophil granulocytes under agarose is simple and inexpensive. Usually results are expressed as the distance migrated by the leading front cellsl, but it is also possible to quantitate number of migrating cells2,3 and observe their morphology4. Finally, modifications have been presented for discriminating chemokinesis (enhanced random migration) from chemotaxis (directed migration) by a checker-board analysis2, or by incorporation of migration stimulating agents, cytotaxins, into the agarose5.
KeywordsNeutrophil Migration Situs Inversus Maximal Cell Density Spontaneous Migration Bacterial Filtrate
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