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Neutrophil Migration and Orientation Under Agarose: Findings in Patients with the Immotile Cilia Syndrome and Effects of Cytochalasin B and Vinblastine

  • Jan Palmblad
  • Ann-Mari Udén
  • Nikos Venizelos
  • Björn Afzelius
  • H. U. Keller
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 141)

Abstract

The technique measuring the migration of PMN neutrophil granulocytes under agarose is simple and inexpensive. Usually results are expressed as the distance migrated by the leading front cellsl, but it is also possible to quantitate number of migrating cells2,3 and observe their morphology4. Finally, modifications have been presented for discriminating chemokinesis (enhanced random migration) from chemotaxis (directed migration) by a checker-board analysis2, or by incorporation of migration stimulating agents, cytotaxins, into the agarose5.

Keywords

Neutrophil Migration Situs Inversus Maximal Cell Density Spontaneous Migration Bacterial Filtrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Jan Palmblad
    • 1
    • 2
  • Ann-Mari Udén
    • 1
    • 2
  • Nikos Venizelos
    • 1
    • 2
  • Björn Afzelius
    • 1
    • 2
  • H. U. Keller
  1. 1.Depts. of Medicine IV & Clin. Bact.the Karolinska Institute at SödersjukhusetStockholmSweden
  2. 2.Wennergren InstituteUniv. of StockholmSweden

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