Advertisement

Nucleotide Concentrations in Leucocytes and Their Use in Controlling the Quality of Cell Preparations

  • R. A. Harkness
  • S. B. Coade
  • M. Mansell
  • R. J. Simmonds
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 141)

Abstract

The association between a reduction in cellular ATP and reduced cell survival has been established for the erythrocyte by many groups (see Seegmiller 1980) and more recently for the kidney (Buhl, 1976a, b). The ATP concentrations in the erythrocyte have guided a search for better condictions of erythrocyte storage for blood transfusion. The factors altering intracellular ATP concentration are therefore of practical interest. A large number of factors reduce ATP concentration but it is difficult to raise it. One condition associated with raised ATP concentration in erythrocytes is renal failure (Mansell and Grimes, 1979).

Keywords

High Pressure Liquid Chromatography Adenine Nucleotide Energy Charge Adenylate Energy Charge Nucleotide Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Atkinson, D.E., 1968, Energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers, Biochemistry, 7: 4030.PubMedCrossRefGoogle Scholar
  2. Buhl, M.R., 1976a, Oxypurine excretion during kidney preservation: an indicator of ischaemic damage, Scand, J. Clin. Lab. Clin. Invest, 36: 169.CrossRefGoogle Scholar
  3. Buhl, M.R., 1976b, The postanoxic regeneration of 5’-nucleotides in rabbit kidney tissue during Zn vitro perfusion, Scand. J. Clib. Lab. Invest, 36: 175.CrossRefGoogle Scholar
  4. Chapman, A.G., Fall, L., and Atkinson, D.E., 1971, Adenylate energy charge in Escherichia colt during growth and starvation, J. Bacteriol, 108: 1072.PubMedGoogle Scholar
  5. Chapman, A.G., and Atkinson, D.E., 1973, Stabilization of the adenylate energy charge by the adenylate deaminase reaction, J. Biol. Chem, 248: 8039.Google Scholar
  6. Dean, B.M., Perrett, D., and Sensi, M., 1978, Changes in nucleotide concentrations in the erythrocytes of man, rabbit and rat during short term storage, Biochem. Biophys. Res. Commn, 80: 147.CrossRefGoogle Scholar
  7. Harkness, R.A., Simmonds, R.J., O’Connor, M.C., and Webster, A.D.B., 1979, Purine metabolism studied with high pressure liquid chromatography, Biochem. Soc. Trans, 7: 1021.PubMedGoogle Scholar
  8. Klebanoff, S.J., and Clark, R.A., 1978, “The Neutrophil: Function and Clinical Disorders”, North Holland, Amsterdam.Google Scholar
  9. Mansell, M., and Grimes, A.J., 1979, Red and white cell abnormalities in renal failure, Brit. J. Haematol, 42: 169.CrossRefGoogle Scholar
  10. McCullough, J., 1977, Liquid preservation of granulocytes for transfusion, Progr. Clin. Biol. Res, 13: 185.Google Scholar
  11. Nussbacher, J., McCullough, J., and Huestis, D., 1977, Granulocyte collection and processing, Progr. Biol. Res, 13: 175.Google Scholar
  12. O’Connor, M.C., Harkness, R.A., Simmonds, R.J., and Hytten, F.E., 1980, The measurements of hypoxanthine, xanthine, inosine and uridine in umbilical cord blood and fetal scalp blood samples as a measure of fetal hypoxia, Brit. J. Obstet. Gynaecol. in press.Google Scholar
  13. Pretlow, T.G., Weir, E.E., and Zettergren, J.G., 1975, Problems connected with the separation of different kinds of cells, Internat. Rev. Exp. Path, 1491.Google Scholar
  14. Schramm, V.L., and Leung, H., 1973, Regulation of adenosine monophosphate levels as a function of adenosine triphosphate and inorganic phosphate - proposed metabolic role for adenosine monophosphate nucleosidase from Azobacter vinelandu, J. Biol. Chem, 248: 8313.PubMedGoogle Scholar
  15. Seegmiller, J.E., 1980, Diseases of purine and pyrimidine metabolism, in: “Metabolic Control and Disease”, P.K. Bondy and L.E. Rosenberg, eds., Saunders, Philadelphia.Google Scholar
  16. Seitz, I.F., 1965, Biochemistry of normal and leukemic leukocytes, thrombocytes and bone marrow cells, Adv. Cancer Res, 9: 304.Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • R. A. Harkness
    • 1
  • S. B. Coade
    • 1
  • M. Mansell
    • 2
  • R. J. Simmonds
    • 1
  1. 1.Divisions of Perinatal MedicineClinical Research CentreMiddxUK
  2. 2.Inherited Metabolic DiseaseClinical Research CentreMiddxUK

Personalised recommendations