Mechanism of Hexose Transport in Human Polymorphonuclear Leukocytes
Cellular functions of human polymorphonuclear leukocytes (PMNL) including phagocytosis, motility, and bactericidal activity require energy derived from glucose. Whereas insulin does not stimulate hexose transport in human PMNL, we have recently reported that chemotactic factors such as complement-derived C5a, the synthetic oligopeptide N-formyl-methionyl-leucyl-phenylalanine (fMLP), or the calcium ionophore A23187 stimulates transport of deoxyglucose (DOG) into human PMNL1,2. FMLP with an EC50 of 1.2 nM, C5a with an EC50 of 1 nM, and A23187 with an EC50 of 10 nM all cause at least a 5-fold stimulation of DOG uptake.
KeywordsArachidonic Acid Calcium Ionophore Arachidonic Acid Metabolism Hexose Transport Calcium Ionophore A23187
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