Physicochemical Surface Changes on Phagocytic Cells during Differentiation in Relation to Chemotaxis and Phagocytosis
Established cell lines with appropriate functional markers for cell differentiation offer simple methods for study of the regulation of cell differentiation. A human promyelocytic leukemia cell line, HL-60, was recently established from the peripheral blood of a patient with acute promyelocytic leukemia1. The majority of the HL-60 cells are promyelocytic in morphology and histochemistry, but 5–10% show morphological characteristics of more mature cells. Induction of morphologic and functional differentiation can be induced by dimethylsulfoxide (DMSO) or other polar solvents1. In addition phorbol diester tumor promoters2 and conditioned media3 can induce differentiation. Several investigations have recently demonstrated that differentiation is accompanied by functional changes such as increased migration and chemotactic responsiveness4, increased oxidative metabolism, phagocytosis and bacterial killing4,5. Furthermore, the cell surface glycoprotein pattern is altered with the expression of a 130.000 dalton glycoprotein6.
KeywordsPhagocytic Cell Phorbol Myristate Acetate DMSO Treatment Human Promyelocytic Leukemia Cell Line Chemotactic Responsiveness
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