Summary
Human eosinophil peroxidase is a cationic protein with a high content of arginine, the enzyme being poorly soluble in water. The purified enzyme is able to carry out the peroxidative chlorination of monochlorodimedon. Like myeloperoxidase the position of the pH optimum of this reaction depends on the ratio of the concentrations of chloride and H2O2. Compared to myeloperoxidase the pH optimum is shifted by 0.8 pH unit to more acid pH values. The physiological consequences of the properties of the eosinophil peroxidase are discussed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
S.L. James and D.G. Colley, Eosinophil-mediated destruction of Schistosoma mansoni eggs, J. Reticuloendothel. Soc. 359 (1976).
J.P. Caulfield, G. Korman, A.E. Butterworth, M. Hogan and J.R. David, The adherence of human neutrophils and eosinophils to schistosomula: Evidence for membrane fusion between cells and parasites, J. Cell Biol. 86: 46 (1980).
J.P. Caulfield, G. Korman, A.E. Butterworth, M. Hogan and J.R. David, Partial and complete detachment of neutrophils and eosinophils from schistosomula: Evidence for the establishment of continuity between a fused and normal parasite membrane, J. Cell Biol. 86: 64 (1980).
P.B. Beeson and D.A. Bass, “The Eosinophil”, vol. 14 in the Series Major Problems in Internal Medicine, L.H. Smith, Jr., ed., W.B. Saunders Comp., Philadelphia (1977).
P.F. Weller and E.J. Goetzl, The human eosinophil, roles in host defense and tissue injury, Am.J. Pathol. 100: 795 (1980).
D.J. McLaren, C.D. Mackenzie, F.J. Ramalho-Pinto, Ultrastructural observations on the in vitro interaction between rat eosinophils and some parasitic helminths (Schistosoma mansoni, Trichinella spiralis and Nippostrongylus braziliensis, Clin. Exp. Immunol. 30: 105 (1977).
K. Agner, Biological effects of hypochlorous acid formed by “MPO”peroxidation in the presence of chloride ions, in: “Structure and function of Oxidation-Reduction Enzymes”, Å. Åkesson and A. Ehrenberg, eds., p. 329, Pergamon Press, Oxford (1970).
T. Stelmaszynska and J.M. Zgliczynski, Myeloperoxidase of human neutrophilic granulocytes as chlorinating enzyme, Eur. J. Biochem. 45: 305 (1974).
J.E. Harrison and J. Schultz, Studies on the chlorinating activity of myeloperoxidase, J. Biol. Chem. 251: 1371 (1976).
H.J. Sips and M.N. Hamers, Mechanism of the bactericidal action of myeloperoxidase: permeabilization of Escherichia colí, Infect. Immun., in press.
R. Weyer, H. Plat and M.N. Hamers, Human eosinophil peroxidase: a novel isolation procedure, spectral properties and chlorinating activity. FEBS Letters, submitted for publication.
R. Wever, M.N. Hamers, R.S.Weening and D. Roos, Characterization of the peroxidase in human eosinophils, Eur. J. Biochem. 108: 491 (1980).
A.R.J. Bakkenist, R. Wever, T. Vulsma, H. Plat and B.F. van Gelder, Isolation procedure and some properties of myeloperoxidase from human leukocytes. Biochim. Biophys. Acta 524: 45 (1978).
I. Olsson, P. Venge, J.K. Spitznagel and R.I. Lehrer, Argininerich cationic proteins of human eosinophil granules, Lab. Invest. 36: 493 (1977).
L.P. Hager, D.R. Morris, F.E. Brown and H. Eberwein, Chloroperoxidase II. utilization of halogen anions, J. Biol. Chem. 241: 1769 (1966).
J.M. Zgliczynski, R.J. Selvaraj, B.B. Paul, T. Stelmaszynska, P.K.F. Poskitt and A.J. Sbarra, Chlorination by the myeloperoxidase-H202-Cl antimicrobial system at acid and neutral pH, Proc. Soc. Exp. Biol. Med. 154: 418 (1977).
A.R.J. Bakkenist, J.E.G. de Boer, H. Plat and R. Wever, The halide complexes of myeloperoxidase and the mechanism of the halogenation reactions, Biochim. Biophys. Acta 613: 337 (1980).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Wever, R., Hamers, M.N., de Graaf, C.J., Weening, R.S., Roos, D., Johnston, R.B. (1982). Characterization of the Peroxidase in Human Eosinophils. In: Rossi, F., Patriarca, P. (eds) Biochemistry and Function of Phagocytes. Advances in Experimental Medicine and Biology, vol 141. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-8088-7_48
Download citation
DOI: https://doi.org/10.1007/978-1-4684-8088-7_48
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-8090-0
Online ISBN: 978-1-4684-8088-7
eBook Packages: Springer Book Archive