Peroxidatic Activity Distinct from Myeloperoxidase in Human Monocytes Cultured in Vitro and in Alveolar Macrophages

  • Janine Breton-Gorius
  • Jean Louis Vildé
  • Josette Guichard
  • William Vainchenker
  • Françoise Basset
  • Charles E. McCall
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 141)


Peroxidase cytochemistry and electron microscopy have revealed that resident macrophages from serous cavities and tissues of several rodent species possess a peroxidatic activity (PA) which is exclusively localized in the rough endoplasmic reticulum (RER) including the nuclear envelope (NE). In contrast, exudate macrophages elicited into the peritoneal cavity with stimulants and which are considered as newly mobilized blood monocytes, only contain PA in granules 1–4. On this basis, Daems and Brederoo4 have suggested the existence of two separate lines of macrophages although Van Furth5 had presented findings showing that resident macrophages also originate from monocytes. More recently, it was reported6–8 that rodent and human monocytes may rapidly develop in vitro after adherence, a PA in RER which resembles that seen in resident macrophages. Moreover, intermediate forms between exudate and resident macrophages have been detected in vivo by several investigators9, 10. All these findings were more consistent with the theory that resident macrophages derive from monocytes. However, macrophages with PA pattern of resident macrophages have not yet been described in man while human monocytes may acquire a PA in RER after adherence.


Alveolar Macrophage Human Monocyte Rough Endoplasmic Reticulum Hairy Cell Leukemia Resident Macrophage 
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  1. 1.
    R.S. Cotran and M. Litt, Ultrastructural localization of horse-radish peroxidase and endogenous peroxidase activity in Guinea-pig peritoneal macrophages. J. Immunol. 105: 1536 (1970).PubMedGoogle Scholar
  2. 2.
    H.D. Fahimi, The fine structural localization of endogenous and exogenous peroxidase activity in Kupffer cells of rat liver. J. Cell Biol. 47: 247 (1970).PubMedCrossRefGoogle Scholar
  3. 3.
    D. Robbins, H.D. Fahimi and R.S. Cotran, Fine structural cytochemical localization of peroxidase activity in rat peritoneal cell mononuclear cells, eosinophils and mast cells. J. Histochem. Cytochem. 19: 751 (1971).CrossRefGoogle Scholar
  4. 4.
    W.Th. Daems and P. Brederoo, Electron microscopical studies on the structure, phagocytic properties and peroxidatic activity of resident and exudate peritoneal macrophages in the Guinea pig. Z. Zellforsch, 144: 247 (1973).PubMedCrossRefGoogle Scholar
  5. 5.
    R. Van Furth, Origin and kinetics of monocytes and macrophages. Sem. Hemat. 7: 125 (1970).Google Scholar
  6. 6.
    P.T. Bodel, B.A. Nichols and D.F. Bainton, Appearance of peroxidase reactivity within the rough endoplasmic reticulum of blood monocytes after surface adherence. J. Exp. Med. 145: 264 (1977).PubMedCrossRefGoogle Scholar
  7. 7.
    R.H.J. Beelen, M.B. Van’t Veer, D.M. Fluitsma, and E.C.M. Hoefsmit, Identification of different peroxidatic activity patterns in human macrophages in vivo and in vitro. J. Reticuloendoth. Soc. 24: 351 (1978).Google Scholar
  8. 8.
    J. Breton-Gorius, J. Guichard, W. Vainchenker and J.L. Vildé, Ultrastructural and cytochemical changes induced by short and prolonged culture of human monocytes. J. Reticuloendoth. Soc. 27: 289 (1980).Google Scholar
  9. 9.
    R.H.J. Beelen, D.M. Broekhuis-Fluitsma, C. Korn and E.C.M. Hoefsmit, Identification of exudate-resident macrophages on the basis of peroxidatic activity. J. Reticuloendoth. Soc. 23: 203 (1978).Google Scholar
  10. 10.
    W. Deimann and H.D. Fahimi, Appearance of transition forms between monocytes and Kupffer cells in the liver of rats treated with glucan. J. Exp. Med. 149: 883 (1979).PubMedCrossRefGoogle Scholar
  11. 11.
    F. Reyes, M.F. Gourdin, J.P. Farcet, B. Dreyfus, and J. Breton-Gorius, Synthesis of a peroxidase activity by cells of Hairy cell leukemia: a study by ultrastructural cytochemistry. Blood, 52: 537 (1978).PubMedGoogle Scholar
  12. 12.
    J.P. Marie, J.P. Vernant, B. Dreyfus, and J. Breton-Gorius, Ultrastructural localization of peroxidases in “undifferentiated” blasts during the blast crisis of chronic granulocytic leukaemia. Brit. J. Haemat. 43: 549 (1979).PubMedCrossRefGoogle Scholar
  13. 13.
    R.C. Graham and M.J. Karnovsky, The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14: 291 (1966).PubMedCrossRefGoogle Scholar
  14. 14.
    F. Roels, E. Wisse, B. De Prest and J. Van der Meulen, Cytochemical discrimination between catalases and peroxidases using diaminobenzidine. Histochem. 41: 281 (1975).CrossRefGoogle Scholar
  15. 15.
    W. Vainchenker, J. Guichard and J. Breton-Gorius, Growth of human megakaryocyte colonies in culture from fetal, neonatal and adult peripheral blood cells: ultrastructural analysis. Blood Cells, 5: 25 (1979).PubMedGoogle Scholar
  16. 16.
    J. Breton-Gorius, Y. Coquin and J. Guichard, Cytochemical distinction between azurophils and catalase-containing granules in leukocytes. I. Studies in developing neutrophils and monocytes from patients with myeloperoxidase deficiency: comparison with peroxidase deficient chicken heterophils. Lab. Invest. 38: 21 (1978).PubMedCrossRefGoogle Scholar
  17. 17.
    F. Gonzalez-Crussi and W. Hsueh, Prostanglandin production by histiocytosis X cells. Fed. Proc. 1038: abstract 4055 (1980)Google Scholar
  18. 18.
    J. Gerrard, J.G. White, G.H.R. Rao and D. Towsend, Localization of platelet prostanglandin production in the platelet dense tubular system. Am. J. Path. 83: 283 (1976).PubMedGoogle Scholar
  19. 19.
    M.R. Soranzo, M.K. Koerten and W.T.H. Daems, Peroxidatic activity and morphometric analysis of alveolar macrophages in the Guinea pig. J. Reticuloendoth. Soc. 23: 343 (1978).Google Scholar
  20. 20.
    R.H.J. Beelen, D.M. Fluitsma, J.W.M. Van der Meer and E.C.M. Hoefsmit, Development of exudate-resident macrophages, on the basis of the pattern of peroxidatic activity in vivo and in vitro. In: “Mononuclear Phagocytes Functional aspects”. Part I. R. Van Furth ed., Martinus Nijhoff publishers. The Hague, Boston, London (1980).Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Janine Breton-Gorius
    • 1
  • Jean Louis Vildé
    • 2
  • Josette Guichard
    • 1
  • William Vainchenker
    • 2
  • Françoise Basset
    • 3
  • Charles E. McCall
  1. 1.INSERM U.91. Hp Henri MondorCréteilFrance
  2. 2.Hp Claude BernardParisFrance
  3. 3.INSERM U.82 CHU Bichat.Paris Cedex 18France

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