Peroxidatic Activity Distinct from Myeloperoxidase in Human Monocytes Cultured in Vitro and in Alveolar Macrophages
Peroxidase cytochemistry and electron microscopy have revealed that resident macrophages from serous cavities and tissues of several rodent species possess a peroxidatic activity (PA) which is exclusively localized in the rough endoplasmic reticulum (RER) including the nuclear envelope (NE). In contrast, exudate macrophages elicited into the peritoneal cavity with stimulants and which are considered as newly mobilized blood monocytes, only contain PA in granules 1–4. On this basis, Daems and Brederoo4 have suggested the existence of two separate lines of macrophages although Van Furth5 had presented findings showing that resident macrophages also originate from monocytes. More recently, it was reported6–8 that rodent and human monocytes may rapidly develop in vitro after adherence, a PA in RER which resembles that seen in resident macrophages. Moreover, intermediate forms between exudate and resident macrophages have been detected in vivo by several investigators9, 10. All these findings were more consistent with the theory that resident macrophages derive from monocytes. However, macrophages with PA pattern of resident macrophages have not yet been described in man while human monocytes may acquire a PA in RER after adherence.
KeywordsAlveolar Macrophage Human Monocyte Rough Endoplasmic Reticulum Hairy Cell Leukemia Resident Macrophage
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