Hydrogen Peroxide Production in A Cell-Free System: Evidence for the Involvement of A Chain Reaction
During the process of phagocytosis, the polymorphonuclear leukocyte undergoes remarkable alterations in cyanide-insensitive oxidative metabolism, collectively referred to as the “respiratory burst.”1 The importance of these events to the bactericidal activity of the cell is underscored by the clinical syndrome, chronic granulomatous disease (CGD). Neutrophils from patients with CGD can ingest bacteria normally or near-normally, but are markedly deficient in their ability to kill most types of engulfed bacteria.2 The biochemical lesion in this disease is characterized by a complete lack of respiratory burst activity.3
KeywordsNADPH Oxidase Respiratory Burst Chronic Granulomatous Disease H202 Production Pyridine Nucleotide
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