Formation of Superoxide Anions and Hydrogen Peroxide by Polymorphonuclear Leukocytes Stimulated with Cytochalasin
Cyanide-insensitive respiration of polymorphonuclear leukocytes is induced by the D or E type of cytochalasins, a family of antibiotics which inhibits various cellular motile functions including membrane movement(Nakagawara et al.,1974). The respiratory stimulation is accompanied by the release of superoxide anions and the activated hexose monophosphate oxidative pathway but without any formation of phagosomes(Nakagawara et al.,1976). The cytochalasins D and E are ideal reagents for studying phagocytotic metabolic changes, because they inhibit endocytotic process at the same time. We are going to show the properties of the burst reactions triggered with cytochalasin D or E and compare the reactions with those occur during the ingestion of bacteria.
KeywordsOxygen Uptake Polymorphonuclear Leukocyte Chronic Granulomatous Disease Superoxide Release Radioactive Iodide
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