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Distribution of Actin-Binding Protein and Myosin in Neutrophils During Chemotaxis and Phagocytosis

  • Niels H. Valerius
  • Olle I. Stendahl
  • John H. Hartwig
  • Thomas P. Stossel
  • H. U. Keller
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 141)

Abstract

Polymorphonuclear leukocytes (PMN) are highly motile cells which are vital for the defense of higher organisms against invasion by microorganisms. Important elements of their function are intimately related to their ability to move. They crawl towards microorganisms, attracted by chemotactic factors, during which process their shape becomes fusifonm or triangular. When they reach their prey they can ingest it by extending pseudopods around it, which eventually fuse to form a closed phagocytic vacuole. During this process the granules are mobilized to get into contact with the plasma membrane to release lysosomal enzymes into the phagosome or to the surrounding medium. All these functions require motility.

Keywords

Actin Filament Contractile Protein Tail Portion Cortical Cytoplasm Yeast Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    D. G. Keyserlingk, Elektronmikroskopische Untersuchung über die Differenzierungsvorgänge im Cytoplasma von segmentierten neutrophilen Leukozyten während der Zellbewegung, Exp. Cell Res. 51: 79 (1968).PubMedCrossRefGoogle Scholar
  2. 2.
    P. L. Moore, H. L. Bank, N. T. Brissie, and S. S. Spicer, Phagocytosis of bacteria by polymorphonuclear leukocytes. A freeze-fracture, scanning electron microscope, and thin section investigation of membrane structure, J. Cell Biol 76: 158 (1978).PubMedCrossRefGoogle Scholar
  3. 3.
    J. M. Oliver, J. A. Krawiec, and E. L. Becker, The distribution of actin during chemotaxis in rabbit neutrophils, J. Reticuloend. Soc. 24: 697 (1978).Google Scholar
  4. 4.
    H. L. Yin and T. P. Stossel, Control of cytoplasmic actin gel-sol transformation by gelsolin, a calcium-dependent regulatory protein, Nature 281: 583 (1979).PubMedCrossRefGoogle Scholar
  5. 5.
    T. P. Stossel and T. D. Pollard, Myosin in polymorphonuclear leukocytes, J. Biol. Chem. 248: 8288 (1973).PubMedGoogle Scholar
  6. 6.
    T. P. Stossel and J. H. Hartwig, Interactions of actin, myosin, and a new actin-binding protein of rabbit pulmonary macrophages. II Role in cytoplasmic movement and phagocytosis, J. Cell Biol. 68: 602 (1976).PubMedCrossRefGoogle Scholar
  7. 7.
    L. A. Boxer and T. P. Stossel, Interactions of actin, myosin and an actin-binding protein of chronic myelogenous leukemia leukocytes, J. Clin. Invest. 57: 964 (1976).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Niels H. Valerius
    • 1
    • 2
    • 3
  • Olle I. Stendahl
    • 1
    • 2
    • 3
  • John H. Hartwig
    • 1
    • 2
    • 3
  • Thomas P. Stossel
    • 1
    • 2
    • 3
  • H. U. Keller
  1. 1.Department of MedicineHematology-Oncology Unit, Massachusetts General Hospital Harvard Medical SchoolBostonUSA
  2. 2.Statens Seruminstitut Department of Clinical MicrobiologyRigshospitalet (State University Hospital)CopenhagenDenmark
  3. 3.Department of Medical MicrobiologyUniversity of LinkopingLinkopingSweden

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