The Secretion of Lysosomal Enzymes by Human Polymorphonuclear Leucocytes (PMN) and Its Modulation by Serum Complement
When engaged in phagocytosis PMN release considerable amounts of their lysosomal constituents. This “regurgitation during feeding” is considered to be caused by passive loss out of incompletely closed phagosomes (1). However, there is an obvious discrepancy between the assumption, that the enzymes are lost during the phagocytic process and the important intraphagosomal function they are thought to have in the destruction of ingested microbes. In this study we investigated the release of lysozyme, myeloperoxidase (MPO) and protease activity at pH=5,5 in relation to the ingestion of E.coli. These enzymes are considered to have important bactericidal as well as digestive functions.
KeywordsLysosomal Enzyme Lysozyme Activity Acid Protease Enzyme Release Passive Loss
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