Relationship between the Enhanced Oxidative Metabolism and the Enhanced Microbicidal Activity of Activated Macrophages

  • Richard B. JohnstonJr.
  • Masataka Sasada
  • Lindsay A. Guthrie
  • Michael J. Pabst
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 141)


Macrophages harvested from animals recently infected by intracellular parasites such as mycobactería exhibit an increase in size and spreading and other manifestations of a state of activation.1,2 Macrophages elicited by injection of inflammatory agents, e.g., lipopolysaccharide (LPS), exhibit most of these properties. Of most direct importance to host defense, infection-activated macrophages have been reported to have an increased capacity to kill certain bacteria, viruses, and protozoa. The interrelationships between macrophage activation, phagocytosis-associated oxidative metabolism, and microbicidal activity have been reviewed recently.3 Certain aspects of these interrelationships will be described in this and the accompanying report.4


Oxidative Metabolism Candida Species Phorbol Myristate Acetate Chronic Granulomatous Disease Phorbol Myristate Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    M. L. Karnovsky and J. K. Lazdins, Biochemical criteria for activated macrophages, J. Immunol. 121: 809 (1978).PubMedGoogle Scholar
  2. 2.
    Z. A. Cohn, The activation of mononuclear phagocytes: fact, fancy, and future, J. Immunol. 121: 813 (1978).PubMedGoogle Scholar
  3. 3.
    R. B. Johnston, Jr., Enhancement of phagocytosis-associated oxidative metabolism as a manifestation of macrophage activation, Lymphokine Reports, in press (1981).Google Scholar
  4. 4.
    N. P. Cummings, M. J. Pabst, and R. B. Johnston, Jr., This volume.Google Scholar
  5. 5.
    R. B. Johnston, Jr., C. A. Godzik, and Z. A. Cohn, Increased superoxide anion production by immunologically activated and chemically elicited macrophages, J. Exp. Med. 148: 115 (1978).PubMedCrossRefGoogle Scholar
  6. 6.
    C. F. Nathan and R. K. Root, Hydrogen peroxide release from mouse peritoneal macrophages: dependence on sequential activation and triggering, J. Exp. Med. 146: 1648 (1977).PubMedCrossRefGoogle Scholar
  7. 7.
    L. C. McPhail, L. R. DeChatelet, and R. B. Johnston, Jr., Generation of chemiluminescence by a particulate fraction isolated from human neutrophils, J. Clin. Invest. 63: 648 (1979).PubMedCrossRefGoogle Scholar
  8. 8.
    R. B. Johnston, Jr., B. B. Keele, Jr., H. P. Misra, J. E. Lehmeyer, L. S. Webb, R. L. Baehner, and K. V. Rajagopalan, The role of superoxide anion generation in phagocytic bactericidal activity, J. Clin. Invest. 55: 1357 (1975).PubMedCrossRefGoogle Scholar
  9. 9.
    A. L. Sagone, Jr., G. W. King, and E. N. Metz, Acomparison of the metabolic response to phagocytosis in human granulocytes and monocytes, J. Clin. Invest. 57: 1352 (1976).PubMedCrossRefGoogle Scholar
  10. 10.
    M. Sasada and R. B. Johnston, Jr., Macrophage microbicidal activity: correlation between phagocytosis-associated oxidative metabolism and the killing of candida by macrophages, J. Exp. Med. 152: 85 (1980).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Richard B. JohnstonJr.
    • 1
  • Masataka Sasada
    • 1
  • Lindsay A. Guthrie
    • 1
  • Michael J. Pabst
    • 1
  1. 1.National Jewish Hospital and Research Center and Departments of Pediatrics and BiochemistryUniversity of Colorado School of MedicineDenverUSA

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