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The Extracellular Stimulation of Intracellular Killing by Phagocytes

  • P. C. J. Leijh
  • Th. L. van Zwet
  • M. Th. van den Barselaar
  • R. van Furth
  • P. Elsbach
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 141)

Abstract

Phagocytes, granulocytes, and mononuclear phagocytes are the major effector cells in the host defence against invading micro-organisms, which these cells ingest, kill intracellularly, and digest. It has been known since the beginning of the century that serum proteins called opsonins are obligatory for optimal ingestion of microorganisms by phagocytes1–4. However, the effect of not only these opsonic but also other serum proteins on the other stages of the phagocytic process, and in particular killing and digestion is less clear. This is due mainly to the fact that until recently it was almost impossible to assess intracellular killing from the continuous ingestion of new live bacteria affecting this measurement5–7. In the present study we used a recently developed method,8,9 to investigate the effect of extracellular stimuli, serum proteins as well as membrane pertubating agents, on the intracellular killing of micro-organisms by granulocytes and monocytes.

Keywords

Phorbol Myristate Acetate Chronic Granulomatous Disease Mononuclear Phagocyte Phorbol Myristate Acetate Calcium Ionophore A23187 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    A.E. Wright, and S.R. Douglas, An experimental investigation of the role of the blood fluids in connection with phagocytosis. Proc. Roy. Soc., London s. B., 72: 357 (1904).Google Scholar
  2. 2.
    T.M. Saba, F.A. Blumenstock, W.A. Scovill, and H. Bernara, Cryoprecipitate reversal of opsonic a2-surface binding glycoprotein deficiency in septic surgical and trauma patients. Science 201: 622 (1978).PubMedCrossRefGoogle Scholar
  3. 3.
    F.M. Griffin, J.A. Griffin, J. Leider, and S.C. Silverstein. Studies on the mechanism of phagocytosis. I. Requirements for circumferential attachment of particle-bound ligands to specific receptors in the macrophage plasma membrane. J. Exp. Med. 142: 1263 (1975).PubMedCrossRefGoogle Scholar
  4. 4.
    F.M. Griffin, J.A. Griffin, and S.C. Silverstein, Studies on the mechanisms of phagocytosis. II. The interaction of macrophages with anti-immunoglobulin IgG-coated bone marrow derived lymphocytes. J. Exp. Med. 144: 788 (1976).PubMedCrossRefGoogle Scholar
  5. 5.
    O. MaalOe. On the relation between alexin and opsonin. Copenhagen, Munksgaard (1946).Google Scholar
  6. 6.
    Z.A. Cohn, and S.I. Morse, Interactions between rabbit polymorphonuclear leucocytes and staphylococci. J. Exp. Med. 110: 419 (1959).PubMedCrossRefGoogle Scholar
  7. 7.
    C.P. Craig, and E. Suter, Extracellular factors influencing staphylocidal capacity of human polymorphonuclear leukocytes. J. Immunol. 97: 287 (1966).PubMedGoogle Scholar
  8. 8.
    P.C.J. Leijh, M.Th. van den Barselaar, Th.L. van Zwet, M.R. Daha, and R. van Furth, Requirement of extra-cellular complement and immunoglobulin for intracellular killing of micro-organisms by human monocytes. J. Clin. Invest. 63: 772 (1979).PubMedCrossRefGoogle Scholar
  9. 9.
    R. van Furth, Th.L. van Zwet, and P.C.J. Leijh, In vitro determination of phagocytosis and intracellular killing by polymorphonuclear and mononuclear phagocytes, in Handbook of Experimental Immunology, D.M. Weir, ed., 3rd ed., ch. 32, Blackwell Scientific Publications, Oxford, London, Edinburgh, Melbourne (1978).Google Scholar
  10. 10.
    A. BOyum, Separation of leucocytes from blood and bone marrow. Scand. J. Lab. Invest. 21 (Suppl.): 97, (1968).Google Scholar
  11. 11.
    P.C.J. Leijh, M.Th. van den Barselaar, and R. van Furth. Manuscript in preparation.Google Scholar
  12. 12.
    W.P. Arend, and M. Mannik, Quantitative studies on IgG receptors on monocytes, in Mononuclear Phagocytes in Immunity, Infection and Pathology, R. van Furth, ed., Blackwell Scientific Publications, Oxford, p. 303 (1975).Google Scholar
  13. 13.
    R.P. Messner, and J. Jelinck, Receptors for human yG on human neutrophils. J. Clin. Invest. 49: 2165 (1970).PubMedCrossRefGoogle Scholar
  14. 14.
    P.C.J. Leijh, Th.L. van Zwet, and R. van Furth, Effect of extracellular serum in the stimulation of intracellular killing of streptococci by human monocytes. Infect. Immun. 30: 421 (1980).PubMedGoogle Scholar
  15. 15.
    E.L. Kaplan, T. Laxdall, and P.G. Quie, Studies of polymorphonuclear leucocytes from patients with chronic granulomatous disease of childhood: Bactericidal capacity for streptococci. Pediatrics 41: 591 (1968).PubMedGoogle Scholar
  16. 16.
    G.L. Mandell, and E.W. Hook, Leucocyte bactericidal activity in chronic granulomatous disease: correlation of bacteria hydrogen peroxide production and susceptibility to intracellular killing. J. Bacterial. 100: 531 (1969).Google Scholar
  17. 17.
    J. Pitt, and H.P. Bernheimer, Role of peroxide in phagocytic killing of pneumococci. Infect. Immun. 9: 48 (1974).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • P. C. J. Leijh
    • 1
  • Th. L. van Zwet
    • 1
  • M. Th. van den Barselaar
    • 1
  • R. van Furth
    • 1
  • P. Elsbach
  1. 1.Department of Infectious DiseasesUniversity HospitalLeidenThe Netherlands

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