Examination of Conditions in Supercritical Fluid Chromatography for Analysis of Ecdysteroids
Ecdysteroids can be chromatographed with a supercritical fluid as the mobile phase in equipment adapted from high pressure liquid chromatography. A variety of columns (both normal and reversed-phase) have been applied under a range of conditions to learn something of the optimum conditions to be used. Temperature had little effect compared to the choice of stationary phase and proportion of methanol in the supercritical fluid. Chromatography was both fast and sensitive but as yet separation is not as efficient as that obtained in liquid chromatography.
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- 1.J. E. Lovelock, A signed and witness statement, unpublished, but illustrated, in: “Modern Supercritical Fluid Chromatography”, C. M. White, ed., VCH, Heidelberg, 1988 (1958).Google Scholar
- 8.M. W. Raynor, J. P. Kithinji, K. D. Bartle, D. E. Games, I. C. Mylchreest, E. D. Morgan, R. Lafont and I. D. Wilson, Packed column supercritical fluid chromatography and linked supercritical-fluid chromatography-mass spectrometry for the analysis of phytoecdysteroids from Silène nutans and Silène otites, J. Chromatog., 467:292–298 (1989).CrossRefGoogle Scholar