A Note on the Regulation of the Corpora Allata by the Brain in the Adult Female American Cockroach, Periplaneta Americana

  • I. Ghanem
  • A. R. McCaffery
Part of the Chromatographic Society Symposium Series book series (CSSS)

Abstract

The corpora allata (CA), the source of juvenile hormones (JH) in insects, play a central role in the regulation of insect metamorphosis and reproduction [1]. The ability of CA to biosynthesize and release JH may be measured in vitro by incubating isolated CA in a defined tissue culture medium in the presence of [methyl-C14]-methionine or [methyl-H3]-methionine. The radiobiosynthesized juvenile hormone is separated on radio-TLC and measured by scintillation counting according to methods described previously [2–4].

Keywords

High Performance liqUid Chromatography Filtration Albumin Acetonitrile Fractionation 

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • I. Ghanem
    • 1
  • A. R. McCaffery
    • 1
  1. 1.Department of Pure and Applied Zoology, School of Animal and Microbial SciencesThe University of ReadingReadingUK

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