Analysis of Gland Secretions of Pentatomoidea (Heteroptera) by Gas Chromatography-Mass Spectrometry Techniques

  • C. E. Evans
  • B. W. Staddon
  • D. E. Games
Part of the Chromatographic Society Symposium Series book series (CSSS)


Results of analyses by gas chromatography and gas chromatography-mass spectrometry on the semiochemicals of several species of the superfamily Pentatomoidea are given. In addition to the primarily defensive metathoracic gland (MG) the four Acanthosomatidae species studied also possess a pair of active dorsal abdominal exocrine glands (DG1) in the adults. The use of microscale hydrogenations in confirming the identity of the DG1 monoterpenes is illustrated. Two methods of sample collection were used, one involving gland dissection under saline into solvent, followed by injection of the sample into the GC, the other involving trapping the volatiles from the bugs onto a porous polymer, followed by their thermal desorption into the gas Chromatograph.


Thermal Desorption Defensive Secretion Scent Gland Airborne Volatile Metathoracic Gland 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    A. J. E. Gough, J. G. C. Hamilton, D. E. Games and B. W. Staddon, Multichemical defense of plant bug Hotea gambiae (Westwood) (Heteroptera: Scutelleridae), J. Chem.Ecol., 11:343–351 (1985).CrossRefGoogle Scholar
  2. 2.
    B. W. Staddon, M. J. Thorne and D. W. Knight, The scent glands and their chemicals in the aposematic cotton harlequin bug, Tectocoris diophthalmus (Heteroptera: Scutelleridae), Australian J. Zool., 35:227–234 (1987).CrossRefGoogle Scholar
  3. 3.
    C. Dupuis, Donnees nouvelles sur la morphologie abdominale des hemipteres, heteropteres et en particulier des Pentatomoidea, Comptes Rendus. XIIIe Congres International de Zoologie, Paris (1948).Google Scholar
  4. 4.
    C. Dupuis, Notes remarques et observations diverses sur les Hemipteres; premiere serie: notes I–IV, III-Dimorphisme sexuel de la glande dorso-abdominale anterieure de certains Asopinae (Pentatomoidae), La Feuille des Naturalistes, 7:1–4 (1952).Google Scholar
  5. 5.
    J. R. Aldrich, N M. S. Blum, H. A. Lloyd and H. M. Fales, Pentatomid natural products chemistry and morphology of III and IV dorsal abdominal glands of adults, J. Chem. Ecol., 4:161–172 (1978).CrossRefGoogle Scholar
  6. 6.
    J. G. C. Hamilton, A. J. E. Gough, B. W. Staddon and D. E. Games, Multichemical defense of plant bug Hotea gambiae (Westwood) (Heteroptera: Scutelleridae) (E)-2-hexenol from abdominal gland in adults, J. Chem. Ecol., 11:1399–1409 (1985).CrossRefGoogle Scholar
  7. 7.
    J. Carayon, Dimorphisme sexuel des glandes, tegumentaires et production de pheromones chez les Hemipteres Pentatomoidea, Comptes Rendus des Seances, Academie des Sciences (Paris) Serie III 299:867–870 (1981).Google Scholar
  8. 8.
    W. C. Mitchell and R. F. L. Mall, Response of the female southern green stink bug and its parasite, Trichopoda pennipes to male stink bug pheromones, J. Economic Entomol., 64:856–859 (1971).Google Scholar
  9. 9.
    S. Moriya and M. Shiga, Attraction of the male brown-winged green bug Plautia stali (Scott) (Heteroptera: Pentatomidae) for males and females of the same species, App. Entomol & Zool. 19:317–322 (1984).Google Scholar
  10. 10.
    D. W. Knight, B. W. Staddon and M. J. Thorne, Presumed sex pheromone from androconial glands of male Cotton Harlequin bug Tectocoris diophthalmus (Heteroptera: Scutelleridae).Identified as 3,5-dihydroxy-4-pyrone, Zeitschrift fur Naturforschung, 40:851–853 (1985).Google Scholar
  11. 11.
    C. Pavis and C. Malosse, Physiologieanimale — mise on evidence d’un attractif sexual produit par les males de Nezara viridula (L) (Heteroptera: Pentatomidae), C.R. Acad. Sci. Paris, Series III, 7:272–276.Google Scholar
  12. 12.
    J. R. Aldrich, J. E. Oliver, W. R. Lusby and J. P. Kochansky, Identification of male specific exocrine secretions from predatory stink bugs (Hemiptera, Pentatomidae), Archives Insect Biochem. & Physiol. 3:1–12 (1986).CrossRefGoogle Scholar
  13. 13.
    M. Borges, P. C. Jepson and P. E. Howse, Long-range mate location and close-range courtship behaviour of the Green Stink Bug, Nezara viridula and its mediation by sex pheromones, Entomol Experimentalis & Applicata, 44:205–212 (1981).CrossRefGoogle Scholar
  14. 14.
    P. Stys and I. Kerzhner, The rank and nomenclature of higher taxa in recent Heteroptera, Acta. Ent Bohemoslov., 72:65–79 (1975).Google Scholar
  15. 15.
    J. H. Cross, A vapor collection and thermal desorption method to measure semiochemical release rates from controlled release formulations, J. Chem. Ecol., 6:781–785 (1980).CrossRefGoogle Scholar
  16. 16.
    C. Kitamura, S. Wakamura and S. Takahashi, Identification and functions of ventral glands secretion of some Heteroptera, App. Entomol. & Zool., 19:33–41 (1984).Google Scholar
  17. 17.
    B. W. Staddon, The scent glands of Heteroptera, Adv. Insect Physiol., 14:351–418 (1979).CrossRefGoogle Scholar
  18. 18.
    D. H. Calam and A. Youdeowei, Identification and functions of secretion from the posterior scent gland of fifth instar larva of the bug Dysdercus intermedins, J. Insect Physiol., 14:1147–1158(1968).Google Scholar
  19. 19.
    I. J. Everton, D. W. Knight and B. W. Staddon, Linalool from the metathoracic scent gland of the cotton stainer Dysdercus intermedins Distant (Heteroptera: Pyrrhocoridae), Comp. Biochem.Physiol., 63B: 157–161 (1979).Google Scholar
  20. 20.
    H. Daroogheh and T. O. Olagbemiro, Linalool from the cotton stainer Dysdercus superstitiosus (F.) (Heteroptera: Pyrrhocoridae), Experientia, 38:421–422 (1982).CrossRefGoogle Scholar
  21. 21.
    J. R. Aldrich, W. R. Lusby, J. P. Kochansky and C. B. Abrams, Volatile Compounds from the predatory insect Podisus maculiventris (Hemiptera: Pentatomoidae): male and female metathoracic scent gland and female dorsal abdominal gland secretions, J. Chem Ecol., 10:561–569 (1984).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • C. E. Evans
    • 1
  • B. W. Staddon
    • 2
  • D. E. Games
    • 1
  1. 1.School of Chemistry and Applied ChemistryUniversity of Wales College of CardiifCardiffUK
  2. 2.School of Pure and Applied BiologyUniversity of Wales College of CardiifCardiffUK

Personalised recommendations