A Note On A Comparative Immunocytochemical Study on the Presence of Cam-HrTH-II- and Lom-Akh-I-Like Molecules in the Central Nervous System of Three Stick Insect Species: Carausius Morosus, Sipyloidea Sipylus and Extatosoma Tiaratum
Antisera directed against the hypertrehalosaemic hormone II of Carausius morosus (Cam-HrTH-II) and against the adipokinetic hormone I of Locusta migratoria (Lom-AKH-I), both members of the AKH/RPCH peptide family, were used to localize immunoreactive molecules in the brain and corpora cardiaca of three species of stick insects. In Carausius as well as in Extatosoma, both antisera stained cells in the glandular part of the corpora cardiaca and axon fibers in the neuropile of the brain. In Sipyloidea however, molecules reactive to Cam-HrTH-II were located in the cells of the glandular part of the corpora cardiaca and in axon fibers of the neuropile of the brain. Lom-AKH-I-like molecules were situated in cells of the pars intercerebralis, in axon fibers and in granules in the storage part of the corpora cardiaca. From the results, obtained by immunostaining on alternating sections and by pre-absorption tests, it seems probable that in Carausius and in Extatosoma, only one type of reactive molecule was present. On the other hand, at least two different molecules must have been present in Sipyloidea, one which was similar to Cam-HrTH-II and another which was similar to Lom-AKH-I.
KeywordsGlandular Cell Stick Insect Peptide Family Fast Atom Bombardment Mass Spectrometry Axon Fiber
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