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Isolation and Structural Characterization of Neuroparsins, Polytropic Neurohormones of the African Locust

  • J. Girardie
  • J. C. Pernollet
  • J. C. Huet
  • A. Girardie
Part of the Chromatographic Society Symposium Series book series (CSSS)

Summary

Neuroparsins A and B, two dimeric sulfur-containing polytropic neurohormones of the African locust, were obtained sufficiently pure to permit their sequencing following two liquid chromatography steps. The N-terminal sequence of neuroparsin B was unique, in contrast to that of neuroparsin A which showed three distinct sequences. The three N-terminal sequences of neuroparsin A included the neuroparsin B sequence itself and extended to five or two supplementary N-terminal residues. The complete sequence of the polypeptide chain of the neuroparsin B homodimer was obtained. It is composed of 78 residues including 2 tyrosines, 1 methionine and 12 cysteines.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • J. Girardie
    • 1
  • J. C. Pernollet
    • 2
  • J. C. Huet
    • 2
  • A. Girardie
    • 1
  1. 1.Laboratoire de Neuroendocrinologie, URA CNRS 1138Université de Bordeaux ITalence CedexFrance
  2. 2.Laboratoire d’Etude des ProtéinesINRAVersailles CedexFrance

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