Summary
Neuroparsins A and B, two dimeric sulfur-containing polytropic neurohormones of the African locust, were obtained sufficiently pure to permit their sequencing following two liquid chromatography steps. The N-terminal sequence of neuroparsin B was unique, in contrast to that of neuroparsin A which showed three distinct sequences. The three N-terminal sequences of neuroparsin A included the neuroparsin B sequence itself and extended to five or two supplementary N-terminal residues. The complete sequence of the polypeptide chain of the neuroparsin B homodimer was obtained. It is composed of 78 residues including 2 tyrosines, 1 methionine and 12 cysteines.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
J. Girardie, D. Bourême, F. Couillaud, M. Tamarelle and A. Girardie, Anti-juvenile effect of neuroparsin A, a neuroprotein isolated from locust corpora cardiaca, Insect Biochem., 17:977–983(1987).
J. Girardie, D. Bourême and A. Girardie, Production sites of the three neurosecretory proteins characterized in the corpora cardiaca of the migratory locust, Insect Biochem., 17:29–36 (1987).
D. Bourême and J. Girardie, Les neuroparsines du criquet (Othoptera): microhétérogénéité d’origine enzymatique, Ann. Soc. Entomol. France, 24:390 (1988).
B. Fournier and J. Girardie, A new function for the locust neuroparsins: stimulation of water reabsorption, J. Insect Physiol., 34:309–313 (1988).
R. Moreau, L. Gourdoux and J. Girardie, Neuroparsin: a new energetic neurohormone in the African locust, Arch. Insect Biochem. Physiol., 8:135–145 (1988).
D. Bourême and J. Girardie, Présence de protéines à ponts disulfure dans les corpora cardiaca d’insectes, Comptes rendus de [Académie des Sciences Paris, 18:655–660.
J. Girardie, A. Faddoul and A. Girardie, Characterization of three neurosecretory proteins from the A median neurosecretory cells of Locusta migratoria by coupled chromatographic, electrophoretic and isoelectrofocusing methods, Insect Biochem., 15:85–92 (1985).
J. Girardie, A. Girardie, J. C. Huet and J. C. Pernollet, Amino acid sequence of locust neuroparsins, Fed. European Biochem. Soc. Lett, 245:4–8 (1989).
W. Mordue, J. P. Morgan and K. J. Sieghert, Strategies for the isolation of insect peptides, Peptides, 6:407–410 (1985).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Plenum Press, New York
About this chapter
Cite this chapter
Girardie, J., Pernollet, J.C., Huet, J.C., Girardie, A. (1990). Isolation and Structural Characterization of Neuroparsins, Polytropic Neurohormones of the African Locust. In: McCaffery, A.R., Wilson, I.D. (eds) Chromatography and Isolation of Insect Hormones and Pheromones. Chromatographic Society Symposium Series. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-8062-7_21
Download citation
DOI: https://doi.org/10.1007/978-1-4684-8062-7_21
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4684-8064-1
Online ISBN: 978-1-4684-8062-7
eBook Packages: Springer Book Archive