Isolation and Structural Characterization of Neuroparsins, Polytropic Neurohormones of the African Locust

  • J. Girardie
  • J. C. Pernollet
  • J. C. Huet
  • A. Girardie
Part of the Chromatographic Society Symposium Series book series (CSSS)


Neuroparsins A and B, two dimeric sulfur-containing polytropic neurohormones of the African locust, were obtained sufficiently pure to permit their sequencing following two liquid chromatography steps. The N-terminal sequence of neuroparsin B was unique, in contrast to that of neuroparsin A which showed three distinct sequences. The three N-terminal sequences of neuroparsin A included the neuroparsin B sequence itself and extended to five or two supplementary N-terminal residues. The complete sequence of the polypeptide chain of the neuroparsin B homodimer was obtained. It is composed of 78 residues including 2 tyrosines, 1 methionine and 12 cysteines.


Polypeptide Chain Cyanogen Bromide Corpus Cardiaca Start Buffer Guanidine Chloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. Girardie, D. Bourême, F. Couillaud, M. Tamarelle and A. Girardie, Anti-juvenile effect of neuroparsin A, a neuroprotein isolated from locust corpora cardiaca, Insect Biochem., 17:977–983(1987).Google Scholar
  2. 2.
    J. Girardie, D. Bourême and A. Girardie, Production sites of the three neurosecretory proteins characterized in the corpora cardiaca of the migratory locust, Insect Biochem., 17:29–36 (1987).CrossRefGoogle Scholar
  3. 3.
    D. Bourême and J. Girardie, Les neuroparsines du criquet (Othoptera): microhétérogénéité d’origine enzymatique, Ann. Soc. Entomol. France, 24:390 (1988).Google Scholar
  4. 4.
    B. Fournier and J. Girardie, A new function for the locust neuroparsins: stimulation of water reabsorption, J. Insect Physiol., 34:309–313 (1988).CrossRefGoogle Scholar
  5. 5.
    R. Moreau, L. Gourdoux and J. Girardie, Neuroparsin: a new energetic neurohormone in the African locust, Arch. Insect Biochem. Physiol., 8:135–145 (1988).CrossRefGoogle Scholar
  6. 6.
    D. Bourême and J. Girardie, Présence de protéines à ponts disulfure dans les corpora cardiaca d’insectes, Comptes rendus de [Académie des Sciences Paris, 18:655–660.Google Scholar
  7. 7.
    J. Girardie, A. Faddoul and A. Girardie, Characterization of three neurosecretory proteins from the A median neurosecretory cells of Locusta migratoria by coupled chromatographic, electrophoretic and isoelectrofocusing methods, Insect Biochem., 15:85–92 (1985).CrossRefGoogle Scholar
  8. 8.
    J. Girardie, A. Girardie, J. C. Huet and J. C. Pernollet, Amino acid sequence of locust neuroparsins, Fed. European Biochem. Soc. Lett, 245:4–8 (1989).CrossRefGoogle Scholar
  9. 9.
    W. Mordue, J. P. Morgan and K. J. Sieghert, Strategies for the isolation of insect peptides, Peptides, 6:407–410 (1985).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • J. Girardie
    • 1
  • J. C. Pernollet
    • 2
  • J. C. Huet
    • 2
  • A. Girardie
    • 1
  1. 1.Laboratoire de Neuroendocrinologie, URA CNRS 1138Université de Bordeaux ITalence CedexFrance
  2. 2.Laboratoire d’Etude des ProtéinesINRAVersailles CedexFrance

Personalised recommendations