Use of Thin-Layer Chromatography, High Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry to Investigate the Relationship Between Juvenile Hormone Titre and Corpus Allatum Activity in Adult Male Periplaneta Americana
Estimates of juvenile hormone (JH) biosynthesis by isolated corpora allata from adult male Periplaneta americana using a non-discriminatory partition assay suggested glandular activity levels that were comparable with the moderate to highly active rates observed in mid-gonotrophic cycle females. Parallel measurements of endogenous whole body JH titres using micro-derivatization, HPLC and GC-MS showed that male cockroaches contained less than 10% of the amount of hormone found in mid-cycle females. Radio-TLC measurements of the short-term in vitro synthetic performance of male glands failed to reveal any major differences from female corpora allata. Follow-up JH titre studies combined with surgical sectioning of the allatal nerves seemed to indicate that the corpora allata of adult male cockroaches are subject to neural inhibition in vivo.
KeywordsJuvenile Hormone Corpus Allatum Juvenile Hormone Titre Methyl Farnesoate Juvenile Hormone Esterase
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