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Use of Thin-Layer Chromatography, High Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry to Investigate the Relationship Between Juvenile Hormone Titre and Corpus Allatum Activity in Adult Male Periplaneta Americana

  • R. J. Weaver
  • J. P. G. Wilkins
  • A. T. Roberts
  • J. P. Edwards
Part of the Chromatographic Society Symposium Series book series (CSSS)

Summary

Estimates of juvenile hormone (JH) biosynthesis by isolated corpora allata from adult male Periplaneta americana using a non-discriminatory partition assay suggested glandular activity levels that were comparable with the moderate to highly active rates observed in mid-gonotrophic cycle females. Parallel measurements of endogenous whole body JH titres using micro-derivatization, HPLC and GC-MS showed that male cockroaches contained less than 10% of the amount of hormone found in mid-cycle females. Radio-TLC measurements of the short-term in vitro synthetic performance of male glands failed to reveal any major differences from female corpora allata. Follow-up JH titre studies combined with surgical sectioning of the allatal nerves seemed to indicate that the corpora allata of adult male cockroaches are subject to neural inhibition in vivo.

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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • R. J. Weaver
    • 1
  • J. P. G. Wilkins
    • 1
  • A. T. Roberts
    • 1
  • J. P. Edwards
    • 1
  1. 1.ADAS Central Science LaboratoryMinistry of Agriculture, Fisheries and FoodSlough, BerkshireUK

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