A Note on Selective Separation of 20,22-Dihydroxyecdysteroids from Insect and Plant Material with Immobilized Phenylboronic Acid
The isolation of ecdysteroids from biological material is a skillful and lengthy process. Short cuts in that isolation, taking advantage of any special properties of ecdysteroids, would be a considerable help in their isolation, identification and quantitative analysis. One possible strategy would be to exploit the presence of vicinal diol systems found in many, if not most ecdysteroids, either at C-2 and C-3, or at C-20 and C-22, or both.
KeywordsHigh Performance Liquid Chromatography Acidic Buffer Organic Modifier Boronic Acid Phenylboronic Acid
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