Thin-Layer Chromatography of Ecdysteroids: Detection and Identification
Recent advances in the instrumentation available for thin-layer chromatography (TLC) which have greatly extended the potential of the technique for ecdysteroid analysis are described. In particular, improvements in scanning densitometers and radioactivity detectors have resulted in a versatile, rapid and quantitative technique which complements other chromatographic methods such as gas and high performance liquid chromatography. In addition, the viability of TLC-mass spectrometry (MS) for ecydsteroids, without prior isolation from the TLC plate, is shown, together with preliminary results for TLC combined with tandem mass-spectrometry (TLC-MS-MS) for studies on phytoecdysteroids.
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