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Thin-Layer Chromatography of Ecdysteroids: Detection and Identification

  • I. D. Wilson
  • R. Lafont
  • C. J. Porter
  • R. G. Kingston
  • K. Longden
  • I. Fleming
  • P. Wall
Part of the Chromatographic Society Symposium Series book series (CSSS)

Summary

Recent advances in the instrumentation available for thin-layer chromatography (TLC) which have greatly extended the potential of the technique for ecdysteroid analysis are described. In particular, improvements in scanning densitometers and radioactivity detectors have resulted in a versatile, rapid and quantitative technique which complements other chromatographic methods such as gas and high performance liquid chromatography. In addition, the viability of TLC-mass spectrometry (MS) for ecydsteroids, without prior isolation from the TLC plate, is shown, together with preliminary results for TLC combined with tandem mass-spectrometry (TLC-MS-MS) for studies on phytoecdysteroids.

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References

  1. 1.
    D. H. S. Horn, The ecdysones, in:“Naturally Occurring Insecticides”, M. Jacobson and D. G. Crosby, eds., Marcel Dekker, New York, pp 333–459 (1971).Google Scholar
  2. 2.
    I. D. Wilson, Reversed-phase thin-layer chromatography of the ecdysteroids, J. Plan. Chromatog., 1:116–122 (1988).Google Scholar
  3. 3.
    I. D. Wilson and S. Lewis, Separation of ecdysteroids by high-performance thin-layer chromatography using automated multiple development, J. Chromatog., 408:445–448 (1987).CrossRefGoogle Scholar
  4. 4.
    I. D. Wilson, R Lafont and P. Wall, TLC of ecdysteroids with off-line identification by fast atom bombardment mass spectrometry directly from the adsorbent, J. Plan. Chromatog., 2:357–359 (1988).Google Scholar
  5. 5.
    I. D. Wilson, Normal-phase thin-layer chromatography on silica gel with simultaneous paraffin impregnation for subsequent reversed-phase thin-layer chromatography in a second dimension, J. Chromatog., 287:183–188 (1984).CrossRefGoogle Scholar
  6. 6.
    R. M. Belchamber, S. J. Brinkworth, H. Read and J. M. Roberts, Qualitative and quantitative image analysis of fluorescents from high-performance thin-layer chromatography, in:“Recent Advances in Thin-Layer Chromatography”, F. A. A. Dallas, H. Read, R. J. Ruane and I. D. Wilson, eds., Plenum, New York, pp 37–44 (1988).CrossRefGoogle Scholar
  7. 7.
    I. D. Wilson and R. Lafont, Thin-layer chromatography and high-performance thin- layer chromatography of [3H] metabolites of 20-hydroxyecdysone, Insect Biochem., 16:33–46 (1986).CrossRefGoogle Scholar
  8. 8.
    P. D. Robinson, E. D. Morgan, I. D. Wilson and R. Lafont, The metabolism of ingested and injected [3H] ecdysone by final instar larvae of Heliothis armigera, Physiol. Entomol., 12:321–330(1987).Google Scholar
  9. 9.
    I. D. Wilson, E. D. Morgan, P. Robinson, R Lafont and C. Biais, A comparison of radio-thin-layer and radio-high-performance liquid chromatography for ecdysteroid metabolism studies, J. Insect Physiol., 34:707–711 (1988).CrossRefGoogle Scholar
  10. 10.
    L. Dinan, Thin-layer chromatography of ecdysone acyl esters and their 2,3-acetonide derivatives, J. Chromatog., 411:379–392 (1987).CrossRefGoogle Scholar
  11. 11.
    G. Dietzel, H. Kubisak and H. Stelzer, Development of 2-dimensional detectors for radio-thin-layer chromatography, in:“Recent Advances in Thin-Layer Chromatography”, F. A. A. Dallas, H. Read, R J. Ruane and I. D. Wilson, eds., Plenum, New York, pp 117–123 (1988).CrossRefGoogle Scholar
  12. 12.
    H. Filthuth, Digital autoradiograph: a new detector for radiochromatography and electrophoresis, in:“Recent Advances in Thin-Layer Chromatography”, F. A. A. Dallas, H. Read, R J. Ruane and I. D. Wilson, eds., Plenum, New York, pp 125–129 (1988).CrossRefGoogle Scholar
  13. 13.
    H. Filthuth, A new detector for radiochromatography and radiolabeled multi sample distributions — the digital autoradiograph, J. Plan. Chromatog., 3:198–202 (1989).Google Scholar
  14. 14.
    I. Smith, The AMBIS two-dimensional Beta scanner, in:“Recent Advances in Thin- Layer Chromatography”, F. A. A. Dallas, H. Read, R J. Ruane and I. D. Wilson, eds., Plenum, New York, pp 77–86 (1988).CrossRefGoogle Scholar
  15. 15.
    M. W. Raynor, J. P. Kithinji, K. D. Bartle, D. E. Games, I. C. Mylchresst, R Lafont, E. D. Morgan and I. D. Wilson, Packed column supercritical fluid chromatography and linked supercritical fluid chromatography-mass spectrometry for the analysis of phytoecdysteroids from Silène nutans and Silène otites, J. Chromatog., 467:292–298 (1989).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • I. D. Wilson
    • 1
  • R. Lafont
    • 2
  • C. J. Porter
    • 3
  • R. G. Kingston
    • 3
  • K. Longden
    • 4
  • I. Fleming
    • 5
  • P. Wall
    • 6
  1. 1.Department of ChemistryUniversity of KeeleKeele, StaffordshireUK
  2. 2.Department de Biologie CNRS-URA 686Ecole Normale SuperieureParis Cedex 05France
  3. 3.V. G. Analytical Ltd.Wythenshawe, ManchesterUK
  4. 4.V. A Howe & Co. Ltd.LondonUK
  5. 5.Camlab Ltd.CambridgeUK
  6. 6.BDH Chemicals Ltd.PooleUK

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