The Effect of Solution Components on Polyacrylamide Gel Formation via Riboflavin-Sensitized Photopolymerization

  • Howard L. Needles
Conference paper


Since Davis (1) first described the use of riboflavin-sensitized photopolymerization of acrylamide-N,N’-methylenebisacrylamide (BIS) mixtures to form large-pore stacking gels for electrophoresis discs, several authors (2-5) have used this photo-polymerization technique to form gels which are useful for various specific separations and applications. Although extensive use of photopolymerized gels in separations has been made, little is known concerning the effect of commonly added components; i.e., accelerators, buffers, viscosity improvers, leading and trailing ions, and proteins, on the rate and course of photopolymerization. Furthermore, Oster (6, 7) has shown that both oxygen and hydrogen donors effect riboflavin-sensitized photopolymerizations of aqueous acrylamide, and we recently reported (8) that both proteins and amino acids have a dramatic effect on riboflavin-sensitized photo-polymerizations. Since workers are often puzzled by their inability to reproducibly and consistently photopolymerize low concentrations of acrylamide-BIS solutions to gels, we have determined the effect of typical solution additives on formation of polyacrylamide gels. Additives, alone and in combination, are shown to have a marked effect on the photopolymerization rate and gelation time of polyacrylamide-BIS gels.


Phosphonium Chloride Photoinitiation System Photopolymerization Rate Canal Industrial Triethanolamine Hydrochloride 
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Copyright information

© Plenum Press, New York 1970

Authors and Affiliations

  • Howard L. Needles
    • 1
  1. 1.Department of Consumer SciencesUniversity of CaliforniaDavisUSA

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