Intracellular pH Modifications Linked to the Activity of the Ferricyanide Driven Activity of the Plasmalemma Redox System in Elodea densa Leaves, Acer pseudoplatanus and Catharanthus roseus Cells
The activity of the ferricyanide-driven redox system at the plasmalemma of plant cells is potentially able to modify the concentration of protons in the cytoplasm. This problem was studied by using the 31P NMR technique to measure the cytoplasmic and vacuolar pH in Elodea densa leaves, Acer pseudoplatanus and Catharanthus roseus cells. Elodea densa leaves (about 2 g FW) or Acer pseudoplatanus and Catharanthus roseus cells (about 7 g FW) were packed in a 20 mm diameter NMR tube and perfused at a rate of 15–30 ml. min−1 with a buffered calcium sulfate solution. Spectra were recorded at 161.93 MHz with a WM 400 Brucker spectrometer by accumulating either 512 scans 1.2 sec each (Acer pseudoplatanus and Catharanthus roseus cells) or 1024 scans (Elodea densa leaves).